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Literature summary for 3.5.4.2 extracted from
- Adenine deaminase of a eukaryotic animal cell, Crithidia fasciculata (1977), Arch. Biochem. Biophys., 183, 7-12.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin protects against heat inactivation in dilute solutions | Crithidia fasciculata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hypoxanthine | competitive, Ki 4 mM | Crithidia fasciculata |  |
| purine | 61% at 0.5 mM | Crithidia fasciculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | 2 | adenine | - |
Crithidia fasciculata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | probably cytosol | Crithidia fasciculata | 5622 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + H2O | Crithidia fasciculata | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.071 | - |
in crude extracts | Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenine + H2O | - |
Crithidia fasciculata | hypoxanthine + NH3 | - |
? | |
| adenine + H2O | - |
Crithidia fasciculata | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
less than 10% loss of activity after 60 min | Crithidia fasciculata |
| 65 | - |
20% loss of activity after 5 min | Crithidia fasciculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | - |
Crithidia fasciculata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5: about 30% of maximal activity, pH 8.5: about 20% of maximal activity | Crithidia fasciculata |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4 | - |
hypoxanthine | competitive | Crithidia fasciculata | |
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