Cloned (Comment) | Organism |
---|---|
expression of the enzyme in Escherichia coli strains MC1061 and BL21(DE3), the His-tagged enzyme constantly shows aggregation after expression | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
purified untagged recombinant enzyme, hanging drop vapour diffusion method, 2 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed in a 1:1 ratio with reservoir solution containing 100 mM HEPES-HCl, pH 7.5, 50 mM CdSO4, 1.6 M sodium acetate, and 10% w/v glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics of the enzyme with bound Zn2+ or with Cd2+ bound at different pH, recombinant enzyme, overview | Methanothermobacter thermautotrophicus | |
0.00075 | - |
1-(5-phosphoribosyl)-AMP | pH 8.0, 65°C, recombinant enzyme with bound Cd2+ | Methanothermobacter thermautotrophicus | |
0.0009 | - |
1-(5-phosphoribosyl)-AMP | pH 8.5, 65°C, recombinant enzyme with bound Cd2+ | Methanothermobacter thermautotrophicus | |
0.0136 | - |
1-(5-phosphoribosyl)-AMP | pH 8.0, 65°C, recombinant enzyme with bound Zn2+ | Methanothermobacter thermautotrophicus | |
0.0204 | - |
1-(5-phosphoribosyl)-AMP | pH 8.5, 65°C, recombinant enzyme with bound Zn2+ | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.95 Cd2+ per enzyme subunit, competes with and replaces Zn2+, binding affects the enzyme structure, three Cd2+ are coordinated by residues Asp85 and Cys86 from one monomer and Cys109 from the other monomer, the fourth Cd2+ is bound by His16 and Asp89 | Methanothermobacter thermautotrophicus | |
Zn2+ | required for activity, zinc-metalloenzyme | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phosphoribosyl)-AMP + H2O | Methanothermobacter thermautotrophicus | the enzyme is involved in histidine biosynthesis | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by anion exchange chromatography and dialysis | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phosphoribosyl)-AMP + H2O | - |
Methanothermobacter thermautotrophicus | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide | - |
ir | |
1-(5-phosphoribosyl)-AMP + H2O | the enzyme is involved in histidine biosynthesis | Methanothermobacter thermautotrophicus | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | the cytoplasmic C-terminus harbors the active site for the histidinol dehydrogenase, EC 1.1.1.23, activity | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
HisI | - |
Methanothermobacter thermautotrophicus |
PR-AMP cyclohydrolase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | assays are performed at 30°C or at 65°C | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
1-(5-phosphoribosyl)-AMP | pH 8.5, 65°C, recombinant enzyme with bound Cd2+ | Methanothermobacter thermautotrophicus | |
0.29 | - |
1-(5-phosphoribosyl)-AMP | pH 8.0, 65°C, recombinant enzyme with bound Cd2+ | Methanothermobacter thermautotrophicus | |
6.4 | - |
1-(5-phosphoribosyl)-AMP | pH 8.0, 65°C, recombinant enzyme with bound Zn2+ | Methanothermobacter thermautotrophicus | |
9.9 | - |
1-(5-phosphoribosyl)-AMP | pH 8.5, 65°C, recombinant enzyme with bound Zn2+ | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | dependent on bound metal ion | Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH profile, recombinant enzyme | Methanothermobacter thermautotrophicus |