Literature summary for 3.5.4.16 extracted from

Kuempornsin, K.; Kotanan, N.; Chobson, P.; Kochakarn, T.; Jirawatcharadech, P.; Jaru-ampornpan, P.; Yuthavong, Y.; Chookajorn, T.
Biochemical and functional characterization of Plasmodium falciparum GTP cyclohydrolase I (2014), Malar. J., 13, 150.

Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)RIL cells	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
H279S	inactive	Plasmodium falciparum
N88Y	the mutant shows about wild type activity	Plasmodium falciparum
R230K	the mutant shows about wild type activity	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
8-oxo-GTP	competitive inhibitor	Plasmodium falciparum
KCl	high salt (1 M KCl) diminishes the activity of the enzyme	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0126	-	GTP	at pH 7.8 and 37°C	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Plasmodium falciparum	-	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	O97071	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-Sepharose column chromatography, and gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Plasmodium falciparum	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?

Synonyms

Synonyms	Comment	Organism
GCH1	-	Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	the enzyme activity is improved by the rise in temperature up to 50°C	Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.039	-	GTP	at pH 7.8 and 37°C	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Plasmodium falciparum

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	11	-	Plasmodium falciparum

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Release 2023.1 (January 2023)