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Literature summary for 3.5.4.16 extracted from
- Characterization of an Fe(2+)-dependent archaeal-specific GTP cyclohydrolase, MptA, from Methanocaldococcus jannaschii (2007), Biochemistry, 46, 6658-6667.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21-Codon Plus (DE3)-PIL | Methanocaldococcus jannaschii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H101N | shows wild type activity | Methanocaldococcus jannaschii |
| H200N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
| H293N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
| H295N | shows reduced enzymatic activity but no reduction in the amount of bound iron | Methanocaldococcus jannaschii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-methyl-GTP | 36% inhibition at 2 mM GMP | Methanocaldococcus jannaschii |  |
| dGTP | 28% inhibition at 2 mM GMP | Methanocaldococcus jannaschii | |
| fapy-GMP | 1 mM results in a 5fold reduction in activity | Methanocaldococcus jannaschii | |
| GMP | 43% inhibition at 2 mM GMP | Methanocaldococcus jannaschii | |
| additional information | Mn2+ enzyme is not inactivated by exposure to oxygen | Methanocaldococcus jannaschii | |
| oxygen | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | dependent, contains 1 Fe2+ per protomer | Methanocaldococcus jannaschii | |
| Mn2+ | at 2 mM Mn2+ is able to restore MptA activity to a higher level than Fe2+, Mn2+ enzyme is not inactivated by exposure to oxygen | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
| 84000 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Methanocaldococcus jannaschii | catalyzes the first step in the biosynthesis of methanopterin | ? | - |
? | |
| additional information | Methanocaldococcus jannaschii MJ0775 | catalyzes the first step in the biosynthesis of methanopterin | ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dithiothreitol | in the absence of dithiothreitol, MptA loses all activity within 4 days at -20°C, this activity can be restored by adding specific divalent metal ions to the oxygen-inactivated enzyme under anaerobic conditions in the presence of dithiothreitol | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
| Methanocaldococcus jannaschii MJ0775 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Mono Q HR column chromatography | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta,gamma-methyleneguanosine 5'-triphosphate + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
| beta,gamma-methyleneguanosine 5'-triphosphate + H2O | - |
Methanocaldococcus jannaschii MJ0775 | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
| GDP + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
| GDP + H2O | - |
Methanocaldococcus jannaschii MJ0775 | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
| GTP + H2O | - |
Methanocaldococcus jannaschii | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine triphosphate | - |
? | |
| GTP + H2O | - |
Methanocaldococcus jannaschii MJ0775 | formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridine triphosphate | - |
? | |
| GTP + H2O | - |
Methanocaldococcus jannaschii | formate + 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate + diphosphate | - |
? | |
| guanosine 5'-[gamma-thio]triphosphate + H2O | - |
Methanocaldococcus jannaschii | dihydroneopterin 2',3'-cyclic phosphate + ? | - |
? | |
| additional information | catalyzes the first step in the biosynthesis of methanopterin | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | no activity towards dGTP, ATP, GMP, ITP, fapy-GTP, or 7-methyl-GTP | Methanocaldococcus jannaschii | ? | - |
? | |
| additional information | catalyzes the first step in the biosynthesis of methanopterin | Methanocaldococcus jannaschii MJ0775 | ? | - |
? | |
| additional information | no activity towards dGTP, ATP, GMP, ITP, fapy-GTP, or 7-methyl-GTP | Methanocaldococcus jannaschii MJ0775 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 37000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GTPCHI | - |
Methanocaldococcus jannaschii |
| MptA | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 100 | relative to a sample of the enzyme heated at 70°C for 10 min, MptA lost 27% activity when it is incubated or 10 min at 80°C, 60% activity at 90°C, and 89% activity at 100°C | Methanocaldococcus jannaschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Methanocaldococcus jannaschii |
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