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Literature summary for 3.5.4.16 extracted from

  • Basset, G.; Quinlivan, E.P.; Ziemak, M.J.; Diaz de la garza, R.; Fischer, M.; Schiffmann, S.; Bacher, A.; Gregory II, J.F.; Hanson, A.D.
    Folate synthesis in plants: the first step of the pterin branch is mediated by a unique bimodular GTP cyclohydrolase I (2002), Proc. Natl. Acad. Sci. USA, 99, 12489-12494.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the yeast expression vector pVT103-U and introduced into yeast strain 971/6a Arabidopsis thaliana
cloned into the yeast expression vector pVT103-U and introduced into yeast strain 971/6a Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
GTP substrate inhibition above 0.1 mM Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
 GTP pH 8.0, 37°C, recombinant enzyme Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Solanum lycopersicum 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000 Solanum lycopersicum
91000
-
 gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Solanum lycopersicum the enzyme mediates the first and committing step of the pterin branch of the folate-synthesis pathway formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O Arabidopsis thaliana the enzyme mediates the first and committing step pf the pterin branch of the folate-synthesis pathway formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O Arabidopsis thaliana the enzyme mediates the first and committing step of the pterin branch of the folate-synthesis pathway formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8S3C2
-
-
Solanum lycopersicum Q8VYU3
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit GCHI mRNA level is highest in unripe fruit Solanum lycopersicum
-
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Arabidopsis thaliana formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O
-
 Solanum lycopersicum formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O the enzyme mediates the first and committing step of the pterin branch of the folate-synthesis pathway Solanum lycopersicum formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O the enzyme mediates the first and committing step pf the pterin branch of the folate-synthesis pathway Arabidopsis thaliana formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O the enzyme mediates the first and committing step of the pterin branch of the folate-synthesis pathway Arabidopsis thaliana formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 50000 Solanum lycopersicum

Synonyms

Synonyms Comment Organism
GCHI
-
 Arabidopsis thaliana
GCHI
-
 Solanum lycopersicum
GTP cyclohydrolase I
-
 Arabidopsis thaliana
GTP cyclohydrolase I
-
 Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.173
-
 GTP pH 8.0, 37°C, recombinant enzyme Solanum lycopersicum