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Literature summary for 3.5.4.15 extracted from
- Plant purine nucleoside catabolism employs a guanosine deaminase required for the generation of xanthosine in Arabidopsis (2013), Plant Cell, 25, 4101-4109.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Nicotiana benthamiana | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.264 | - |
guanosine | at pH 8.0 and 40°C | Arabidopsis thaliana |  |
| 0.5761 | - |
2'-deoxyguanosine | at pH 8.0 and 40°C | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyguanosine + H2O | Arabidopsis thaliana | - |
2'-deoxyxanthosine + NH3 | - |
? | |
| guanosine + H2O | Arabidopsis thaliana | - |
xanthosine + NH3 | - |
? | |
| additional information | Arabidopsis thaliana | no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Col-0 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity column chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyguanosine + H2O | - |
Arabidopsis thaliana | 2'-deoxyxanthosine + NH3 | - |
? | |
| guanosine + H2O | - |
Arabidopsis thaliana | xanthosine + NH3 | - |
? | |
| additional information | no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GSDA | - |
Arabidopsis thaliana |
| guanosine deaminase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.611 | - |
2'-deoxyguanosine | at pH 8.0 and 40°C | Arabidopsis thaliana | |
| 1.753 | - |
guanosine | at pH 8.0 and 40°C | Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.061 | - |
2'-deoxyguanosine | at pH 8.0 and 40°C | Arabidopsis thaliana | |
| 6.642 | - |
guanosine | at pH 8.0 and 40°C | Arabidopsis thaliana | |
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Release 2023.1 (January 2023)
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