Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cyanophage S-TIM5 |
Crystallization (Comment) | Organism |
---|---|
bound to dTTP, hanging drop vapor diffusion method, using 0.2 M potassium formate, 20% (w/v) PEG 3350 | Cyanophage S-TIM5 |
Protein Variants | Comment | Organism |
---|---|---|
N43G | the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type | Cyanophage S-TIM5 |
T61A | the mutant shows about 65% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
T61D | the mutant shows about 7% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
T61S | the mutant shows about 145% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
T61V | the mutant shows about 50% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
W42A | inactive | Cyanophage S-TIM5 |
W42F | the mutant shows about 118% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
W42Y | the mutant shows about wild type activity | Cyanophage S-TIM5 |
Y116E | inactive | Cyanophage S-TIM5 |
Y116F | the mutant shows about 140% activity compared to the wild type enzyme | Cyanophage S-TIM5 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | - |
Cyanophage S-TIM5 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Cyanophage S-TIM5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | Cyanophage S-TIM5 | - |
dUMP + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyanophage S-TIM5 | H6WFU3 | - |
- |
Purification (Comment) | Organism |
---|---|
HisPur cobalt resin column chromatography and Superdex 200 gel filtration | Cyanophage S-TIM5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dCMP + H2O | - |
Cyanophage S-TIM5 | dUMP + NH3 | - |
? | |
additional information | no deamination activity with dCTP or CMP | Cyanophage S-TIM5 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DCD | - |
Cyanophage S-TIM5 |
deoxycytidylate deaminase | - |
Cyanophage S-TIM5 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cyanophage S-TIM5 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | more than 80% activity between pH 6.0 and 8.0 | Cyanophage S-TIM5 |