BRENDA - Enzyme Database
show all sequences of 3.5.4.12

A tale of two enzymes, deoxycytidylate deaminase and thymidylate synthase

Maley, F.; Maley, G.F.; Prog. Nucleic Acid Res. Mol. Biol. 39, 49-80 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dCTP
-
Bacillus subtilis
dCTP
dCTP promotes an aggregation of enzyme subunits to the active state; positive allosteric effector
Enterobacteria phage T2
dCTP
positive allosteric effector
Enterobacteria phage T6
dCTP
positive allosteric effector
Equus asinus
dCTP
positive allosteric effector
Escherichia virus T4
dCTP
activation by dCTP is reversed by addition of dTTP; dCTP promotes an aggregation of enzyme subunits to the active state; positive allosteric effector
Gallus gallus
dCTP
-
Herpes simplex virus
dCTP
activation by dCTP is reversed by addition of dTTP; positive allosteric effector
Homo sapiens
dCTP
positive allosteric effector
Rattus norvegicus
dCTP
-
Saccharomyces cerevisiae
Application
Application
Commentary
Organism
medicine
associated mainly with dividing tissues, potential diagnostic tool for various disease states in which enzyme is elevated
Homo sapiens
pharmacology
might have applications in cancer chemotherapy. Enzyme may be an inhibitor target for antitumor agents
Equus asinus
pharmacology
enzyme might be a reasonable target for chemotherapeutic agents directed against parasitic as well as neoplastic diseases by limiting the synthesis of dUMP, particularly when used in combination with inhibitors of dTMP synthase or other purine and pyrimidine inhibitors of DNA synthesis
Homo sapiens
Cloned(Commentary)
Commentary
Organism
-
Escherichia virus T4
Crystallization (Commentary)
Crystallization
Organism
-
Enterobacteria phage T2
General Stability
General Stability
Organism
dCTP stabilizes
Gallus gallus
dCTP stabilizes
Escherichia virus T4
dCTP stabilizes
Enterobacteria phage T2
dCTP stabilizing hexameric state, dTTP destabilizing
Equus asinus
dCTP stabilizing hexameric state, dTTP destabilizing
Enterobacteria phage T2
enzyme is unstable
Gallus gallus
Inhibitors
Inhibitors
Commentary
Organism
Structure
dTTP
allosteric inhibitor; dTTP favores disaggregated or inhibited state
Enterobacteria phage T2
dTTP
allosteric inhibitor
Enterobacteria phage T6
dTTP
allosteric inhibitor
Equus asinus
dTTP
allosteric inhibitor
Escherichia virus T4
dTTP
allosteric inhibitor; dTTP favores disaggregated or inhibited state
Gallus gallus
dTTP
-
Herpes simplex virus
dTTP
allosteric inhibitor
Homo sapiens
dTTP
allosteric inhibitor
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
additional information
-
additional information
-
Enterobacteria phage T2
additional information
-
additional information
-
Equus asinus
additional information
-
additional information
-
Escherichia virus T4
additional information
-
additional information
-
Gallus gallus
additional information
-
additional information
-
Homo sapiens
additional information
-
additional information
-
Rattus norvegicus
additional information
-
additional information
-
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Gallus gallus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Bacillus subtilis
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Homo sapiens
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
-
dCMP + H2O
Rattus norvegicus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Saccharomyces cerevisiae
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Escherichia virus T4
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Sea urchin
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Equus asinus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Herpes simplex virus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T2
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Macaca mulatta polyomavirus 1
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T6
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Polyomavirus sp.
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Gallus gallus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Homo sapiens
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
-
dCMP + H2O
Rattus norvegicus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Saccharomyces cerevisiae
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Escherichia virus T4
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Sea urchin
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Equus asinus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Herpes simplex virus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T2
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Macaca mulatta polyomavirus 1
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T6
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Polyomavirus sp.
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
additional information
Homo sapiens
-
?
-
-
-
additional information
Escherichia virus T4
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Enterobacteria phage T2
-
T2-phage infected Escherichia coli; T2r+-infected Escherichia coli B; T2-, T4-, and T6-phage induced enzyme in Escherichia coli
-
Enterobacteria phage T6
-
T2-, T4-, and T6-phage induced enzyme in Escherichia coli
-
Equus asinus
-
donkey
-
Escherichia virus T4
-
T2-, T4-, and T6-phage induced enzyme in Escherichia coli; T4-phage infected Escherichia coli
-
Gallus gallus
-
-
-
Herpes simplex virus
-
induced by
-
Homo sapiens
-
human
-
Macaca mulatta polyomavirus 1
-
induced by
-
no activity in Escherichia coli
-
enzyme not detectable in Escherichia coli until after infection with T-even bacteriophage; wild type Escherichia coli contains no dCMP deaminase
-
no activity in Salmonella typhimurium
-
do not contain dCMP deaminase
-
Polyomavirus sp.
-
induced by
-
Rattus norvegicus
-
-
-
Saccharomyces cerevisiae
-
-
-
Sea urchin
-
-
-
Purification (Commentary)
Commentary
Organism
-
Gallus gallus
HeLa cells
Homo sapiens
Reaction
Reaction
Commentary
Organism
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Bacillus subtilis
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T2
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Enterobacteria phage T6
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Equus asinus
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Escherichia virus T4
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Gallus gallus
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Homo sapiens
dCMP + H2O = dUMP + NH3
feedback regulation; highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
egg
-
Sea urchin
-
embryo
-
Gallus gallus
-
embryo
-
Rattus norvegicus
-
HeLa cell
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
additional information
associated mainly with dividing tissues
Bacillus subtilis
-
additional information
associated mainly with dividing tissues
Enterobacteria phage T2
-
additional information
associated mainly with dividing tissues
Enterobacteria phage T6
-
additional information
associated mainly with dividing tissues
Equus asinus
-
additional information
associated mainly with dividing tissues
Escherichia virus T4
-
additional information
associated mainly with dividing tissues
Gallus gallus
-
additional information
associated mainly with dividing tissues
Herpes simplex virus
-
additional information
associated mainly with dividing tissues
Homo sapiens
-
additional information
associated mainly with dividing tissues
Macaca mulatta polyomavirus 1
-
additional information
associated mainly with dividing tissues
Polyomavirus sp.
-
additional information
associated mainly with dividing tissues
Rattus norvegicus
-
additional information
associated mainly with dividing tissues
Saccharomyces cerevisiae
-
additional information
associated mainly with dividing tissues
Sea urchin
-
spleen
-
Equus asinus
-
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
500
-
HeLa cells
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-bromo-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
-
289020
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
-
289020
Enterobacteria phage T2
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
very good substrate
289020
Gallus gallus
5-bromo-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289020
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
very good substrate
289020
Gallus gallus
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
very good substrate
289020
Enterobacteria phage T2
5-fluoro-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289020
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289020
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Gallus gallus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Escherichia virus T4
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289020
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289020
Gallus gallus
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289020
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
very poor substrate
289020
Saccharomyces cerevisiae
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
very poor substrate
289020
Enterobacteria phage T2
5-iodo-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
very good substrate
289020
Gallus gallus
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
very good substrate
289020
Enterobacteria phage T2
5-methyl-dUMP + NH3
-
-
-
?
dCMP + H2O
-
289020
Gallus gallus
dUMP + NH3
-
289020
Gallus gallus
?
dCMP + H2O
-
289020
Bacillus subtilis
dUMP + NH3
-
289020
Bacillus subtilis
?
dCMP + H2O
-
289020
Homo sapiens
dUMP + NH3
-
289020
Homo sapiens
-
dCMP + H2O
-
289020
Rattus norvegicus
dUMP + NH3
-
289020
Rattus norvegicus
?
dCMP + H2O
-
289020
Saccharomyces cerevisiae
dUMP + NH3
-
289020
Saccharomyces cerevisiae
?
dCMP + H2O
-
289020
Escherichia virus T4
dUMP + NH3
-
289020
Escherichia virus T4
?
dCMP + H2O
-
289020
Sea urchin
dUMP + NH3
-
289020
Sea urchin
?
dCMP + H2O
-
289020
Equus asinus
dUMP + NH3
-
289020
Equus asinus
?
dCMP + H2O
-
289020
Herpes simplex virus
dUMP + NH3
-
289020
Herpes simplex virus
?
dCMP + H2O
-
289020
Enterobacteria phage T2
dUMP + NH3
-
289020
Enterobacteria phage T2
?
dCMP + H2O
-
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
289020
Macaca mulatta polyomavirus 1
?
dCMP + H2O
-
289020
Enterobacteria phage T6
dUMP + NH3
-
289020
Enterobacteria phage T6
?
dCMP + H2O
-
289020
Polyomavirus sp.
dUMP + NH3
-
289020
Polyomavirus sp.
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Gallus gallus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Bacillus subtilis
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Homo sapiens
dUMP + NH3
-
-
-
-
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Rattus norvegicus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Escherichia virus T4
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Sea urchin
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Equus asinus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Herpes simplex virus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Enterobacteria phage T6
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Polyomavirus sp.
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Gallus gallus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Bacillus subtilis
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Homo sapiens
dUMP + NH3
-
-
-
-
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Rattus norvegicus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Escherichia virus T4
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Sea urchin
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Equus asinus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Herpes simplex virus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Enterobacteria phage T6
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Polyomavirus sp.
dUMP + NH3
-
-
-
?
additional information
-
289020
Homo sapiens
?
-
-
-
-
additional information
substrate specificity
289020
Gallus gallus
?
-
-
-
-
additional information
substrate specificity
289020
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
289020
Homo sapiens
?
-
-
-
-
additional information
substrate specificity
289020
Saccharomyces cerevisiae
?
-
-
-
-
additional information
substrate specificity
289020
Equus asinus
?
-
-
-
-
additional information
substrate specificity
289020
Enterobacteria phage T2
?
-
-
-
-
additional information
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
289020
Escherichia virus T4
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 identical subunits
Enterobacteria phage T2
hexamer
6 identical subunits
Enterobacteria phage T6
hexamer
6 identical subunits
Equus asinus
hexamer
6 identical subunits
Escherichia virus T4
hexamer
6 identical subunits
Gallus gallus
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 identical subunits
Homo sapiens
hexamer
6 identical subunits
Rattus norvegicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Escherichia virus T4
30
-
optimum condition
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dCTP
-
Bacillus subtilis
dCTP
dCTP promotes an aggregation of enzyme subunits to the active state; positive allosteric effector
Enterobacteria phage T2
dCTP
positive allosteric effector
Enterobacteria phage T6
dCTP
positive allosteric effector
Equus asinus
dCTP
positive allosteric effector
Escherichia virus T4
dCTP
activation by dCTP is reversed by addition of dTTP; dCTP promotes an aggregation of enzyme subunits to the active state; positive allosteric effector
Gallus gallus
dCTP
-
Herpes simplex virus
dCTP
activation by dCTP is reversed by addition of dTTP; positive allosteric effector
Homo sapiens
dCTP
positive allosteric effector
Rattus norvegicus
dCTP
-
Saccharomyces cerevisiae
Application (protein specific)
Application
Commentary
Organism
medicine
associated mainly with dividing tissues, potential diagnostic tool for various disease states in which enzyme is elevated
Homo sapiens
pharmacology
might have applications in cancer chemotherapy. Enzyme may be an inhibitor target for antitumor agents
Equus asinus
pharmacology
enzyme might be a reasonable target for chemotherapeutic agents directed against parasitic as well as neoplastic diseases by limiting the synthesis of dUMP, particularly when used in combination with inhibitors of dTMP synthase or other purine and pyrimidine inhibitors of DNA synthesis
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Escherichia virus T4
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Enterobacteria phage T2
General Stability (protein specific)
General Stability
Organism
dCTP stabilizes
Gallus gallus
dCTP stabilizes
Escherichia virus T4
dCTP stabilizes
Enterobacteria phage T2
dCTP stabilizing hexameric state, dTTP destabilizing
Equus asinus
dCTP stabilizing hexameric state, dTTP destabilizing
Enterobacteria phage T2
enzyme is unstable
Gallus gallus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dTTP
allosteric inhibitor; dTTP favores disaggregated or inhibited state
Enterobacteria phage T2
dTTP
allosteric inhibitor
Enterobacteria phage T6
dTTP
allosteric inhibitor
Equus asinus
dTTP
allosteric inhibitor
Escherichia virus T4
dTTP
allosteric inhibitor; dTTP favores disaggregated or inhibited state
Gallus gallus
dTTP
-
Herpes simplex virus
dTTP
allosteric inhibitor
Homo sapiens
dTTP
allosteric inhibitor
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
additional information
-
additional information
-
Enterobacteria phage T2
additional information
-
additional information
-
Equus asinus
additional information
-
additional information
-
Escherichia virus T4
additional information
-
additional information
-
Gallus gallus
additional information
-
additional information
-
Homo sapiens
additional information
-
additional information
-
Rattus norvegicus
additional information
-
additional information
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Gallus gallus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Bacillus subtilis
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Homo sapiens
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
-
dCMP + H2O
Rattus norvegicus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Saccharomyces cerevisiae
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Escherichia virus T4
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Sea urchin
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Equus asinus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Herpes simplex virus
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T2
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Macaca mulatta polyomavirus 1
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T6
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Polyomavirus sp.
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
dUMP + NH3
-
-
?
dCMP + H2O
Gallus gallus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Homo sapiens
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
-
dCMP + H2O
Rattus norvegicus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Saccharomyces cerevisiae
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Escherichia virus T4
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Sea urchin
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Equus asinus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Herpes simplex virus
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T2
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Macaca mulatta polyomavirus 1
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Enterobacteria phage T6
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
dCMP + H2O
Polyomavirus sp.
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
-
?
additional information
Homo sapiens
-
?
-
-
-
additional information
Escherichia virus T4
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gallus gallus
HeLa cells
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
egg
-
Sea urchin
-
embryo
-
Gallus gallus
-
embryo
-
Rattus norvegicus
-
HeLa cell
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
additional information
associated mainly with dividing tissues
Bacillus subtilis
-
additional information
associated mainly with dividing tissues
Enterobacteria phage T2
-
additional information
associated mainly with dividing tissues
Enterobacteria phage T6
-
additional information
associated mainly with dividing tissues
Equus asinus
-
additional information
associated mainly with dividing tissues
Escherichia virus T4
-
additional information
associated mainly with dividing tissues
Gallus gallus
-
additional information
associated mainly with dividing tissues
Herpes simplex virus
-
additional information
associated mainly with dividing tissues
Homo sapiens
-
additional information
associated mainly with dividing tissues
Macaca mulatta polyomavirus 1
-
additional information
associated mainly with dividing tissues
Polyomavirus sp.
-
additional information
associated mainly with dividing tissues
Rattus norvegicus
-
additional information
associated mainly with dividing tissues
Saccharomyces cerevisiae
-
additional information
associated mainly with dividing tissues
Sea urchin
-
spleen
-
Equus asinus
-
spleen
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
500
-
HeLa cells
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-bromo-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
-
289020
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
-
289020
Enterobacteria phage T2
5-bromo-dUMP + NH3
-
-
-
?
5-bromo-dCMP + H2O
very good substrate
289020
Gallus gallus
5-bromo-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289020
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
very good substrate
289020
Gallus gallus
5-fluoro-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
very good substrate
289020
Enterobacteria phage T2
5-fluoro-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289020
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289020
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Gallus gallus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Escherichia virus T4
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289020
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289020
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289020
Gallus gallus
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289020
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
very poor substrate
289020
Saccharomyces cerevisiae
5-iodo-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
very poor substrate
289020
Enterobacteria phage T2
5-iodo-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Saccharomyces cerevisiae
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289020
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
very good substrate
289020
Gallus gallus
5-methyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
very good substrate
289020
Enterobacteria phage T2
5-methyl-dUMP + NH3
-
-
-
?
dCMP + H2O
-
289020
Gallus gallus
dUMP + NH3
-
289020
Gallus gallus
?
dCMP + H2O
-
289020
Bacillus subtilis
dUMP + NH3
-
289020
Bacillus subtilis
?
dCMP + H2O
-
289020
Homo sapiens
dUMP + NH3
-
289020
Homo sapiens
-
dCMP + H2O
-
289020
Rattus norvegicus
dUMP + NH3
-
289020
Rattus norvegicus
?
dCMP + H2O
-
289020
Saccharomyces cerevisiae
dUMP + NH3
-
289020
Saccharomyces cerevisiae
?
dCMP + H2O
-
289020
Escherichia virus T4
dUMP + NH3
-
289020
Escherichia virus T4
?
dCMP + H2O
-
289020
Sea urchin
dUMP + NH3
-
289020
Sea urchin
?
dCMP + H2O
-
289020
Equus asinus
dUMP + NH3
-
289020
Equus asinus
?
dCMP + H2O
-
289020
Herpes simplex virus
dUMP + NH3
-
289020
Herpes simplex virus
?
dCMP + H2O
-
289020
Enterobacteria phage T2
dUMP + NH3
-
289020
Enterobacteria phage T2
?
dCMP + H2O
-
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
289020
Macaca mulatta polyomavirus 1
?
dCMP + H2O
-
289020
Enterobacteria phage T6
dUMP + NH3
-
289020
Enterobacteria phage T6
?
dCMP + H2O
-
289020
Polyomavirus sp.
dUMP + NH3
-
289020
Polyomavirus sp.
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Gallus gallus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Bacillus subtilis
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Homo sapiens
dUMP + NH3
-
-
-
-
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Rattus norvegicus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Escherichia virus T4
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Sea urchin
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Equus asinus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Herpes simplex virus
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Enterobacteria phage T6
dUMP + NH3
-
-
-
?
dCMP + H2O
providing an adequate supply of dUMP for formation of dTMP, via dTMP synthase a crucial intermediate for synthesis of DNA
289020
Polyomavirus sp.
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Gallus gallus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Bacillus subtilis
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Homo sapiens
dUMP + NH3
-
-
-
-
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Rattus norvegicus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Saccharomyces cerevisiae
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Escherichia virus T4
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Sea urchin
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Equus asinus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Herpes simplex virus
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Enterobacteria phage T2
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Macaca mulatta polyomavirus 1
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Enterobacteria phage T6
dUMP + NH3
-
-
-
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289020
Polyomavirus sp.
dUMP + NH3
-
-
-
?
additional information
-
289020
Homo sapiens
?
-
-
-
-
additional information
substrate specificity
289020
Gallus gallus
?
-
-
-
-
additional information
substrate specificity
289020
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
289020
Homo sapiens
?
-
-
-
-
additional information
substrate specificity
289020
Saccharomyces cerevisiae
?
-
-
-
-
additional information
substrate specificity
289020
Equus asinus
?
-
-
-
-
additional information
substrate specificity
289020
Enterobacteria phage T2
?
-
-
-
-
additional information
function of enzyme is that of enhancing stability of T4-phage multienzyme complex involved in dNTP synthesis, the complex is less stable in cd mutants
289020
Escherichia virus T4
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 identical subunits
Enterobacteria phage T2
hexamer
6 identical subunits
Enterobacteria phage T6
hexamer
6 identical subunits
Equus asinus
hexamer
6 identical subunits
Escherichia virus T4
hexamer
6 identical subunits
Gallus gallus
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 identical subunits
Homo sapiens
hexamer
6 identical subunits
Rattus norvegicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Escherichia virus T4
30
-
optimum condition
Homo sapiens
Other publictions for EC 3.5.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734322
Marx
The first crystal structure of ...
Cyanophage S-TIM5
J. Biol. Chem.
290
682-690
2015
-
-
1
1
10
-
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
734401
Xu
STRIPE2 encodes a putative dCM ...
Oryza sativa
J. Genet. Genomics
41
539-548
2014
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712083
Jansen
Deoxyuridine analog nucleotide ...
Homo sapiens
Fundam. Clin. Pharmacol.
25
172-185
2011
1
-
-
-
-
-
8
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711628
Li
DNA mismatch repair (MMR)-depe ...
Homo sapiens
Br. J. Pharmacol.
158
679-692
2009
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688405
Hou
Crystal structures of Streptoc ...
Streptococcus mutans
J. Mol. Biol.
377
220-231
2008
1
-
1
1
-
-
1
-
-
5
-
1
-
2
-
-
1
-
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
5
-
1
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686248
Liskay
Involvement of deoxycytidylate ...
Saccharomyces cerevisiae
Curr. Biol.
17
R755-R757
2007
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688732
Zhang
Chloroviruses encode a bifunct ...
Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
2007
1
-
1
-
-
-
3
2
-
5
1
2
-
4
-
-
1
-
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
2
-
5
1
2
-
-
-
1
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
-
-
-
667587
Almog
Three-dimensional structure of ...
Escherichia virus T4
Biochemistry
43
13715-13723
2004
1
1
1
1
1
-
2
-
-
1
-
1
-
1
-
-
1
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
1
1
-
-
2
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669680
Gribaudo
Human cytomegalovirus requires ...
Homo sapiens
J. Gen. Virol.
84
1437-1441
2003
1
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670328
Liou
Assessment of the effect of ph ...
Homo sapiens
Mol. Pharmacol.
63
105-110
2003
-
-
-
-
-
-
10
1
-
1
-
1
-
3
-
-
1
-
-
1
-
-
4
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
-
10
6
1
-
1
-
1
-
-
-
1
-
1
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
667541
Cha
Modifications of deoxycytidine ...
Rattus norvegicus
Biochem. Pharmacol.
63
717-723
2002
1
-
-
-
-
-
1
4
-
-
-
1
-
1
-
-
-
-
-
3
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
-
3
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668584
Jost
5-Methyldeoxycytidine monophos ...
Homo sapiens
FEBS Lett.
519
128-134
2002
1
-
-
-
-
-
1
1
-
1
-
3
-
2
-
-
1
-
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
3
-
-
-
1
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289015
Keefe
A T4-phage deoxycytidylate dea ...
Enterobacteria phage T2, Enterobacteria phage T6, Escherichia virus T4
J. Biol. Chem.
275
12598-12602
2000
7
-
1
-
3
4
4
5
-
4
9
2
-
4
-
-
1
3
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
7
-
1
-
-
3
4
-
4
-
5
-
4
9
2
-
-
-
1
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
-
-
-
289014
Nucci
Interaction of the high-affini ...
Equus asinus
Arch. Biochem. Biophys.
310
49-53
1994
2
1
-
-
-
-
3
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289017
Moore
Identification of a site neces ...
Escherichia virus T4, Homo sapiens
Biochemistry
33
2104-2112
1994
3
-
2
-
1
-
6
-
-
1
-
2
-
2
-
-
1
2
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
1
-
-
6
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
289018
Maley
Properties of an affinity-colu ...
Homo sapiens
Biochim. Biophys. Acta
1162
161-170
1993
2
-
1
-
-
5
11
-
-
7
2
1
-
3
-
-
1
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
2
-
1
-
-
-
5
-
11
-
-
-
7
2
1
-
-
-
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
289016
Xu
Modulation of deoxycytidylate ...
Homo sapiens
Biochem. Pharmacol.
44
1819-1827
1992
1
1
-
-
-
-
7
-
-
-
-
2
-
2
-
-
1
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
-
-
-
2
-
-
-
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289012
Nucci
Allosteric modifier and substr ...
Equus asinus
Arch. Biochem. Biophys.
289
19-25
1991
2
-
-
-
-
1
4
1
-
-
2
1
-
3
-
-
1
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
289013
Whitehead
Hill coefficient ratios give b ...
Equus asinus
Arch. Biochem. Biophys.
289
12-18
1991
2
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289019
Riva
-
Correlation between deoxycytid ...
Homo sapiens
J. Cell. Pharmacol.
1
79-85
1990
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289020
Maley
A tale of two enzymes, deoxycy ...
Bacillus subtilis, Enterobacteria phage T2, Enterobacteria phage T6, Equus asinus, Escherichia virus T4, Gallus gallus, Herpes simplex virus, Homo sapiens, Macaca mulatta polyomavirus 1, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Saccharomyces cerevisiae, Sea urchin
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
10
3
1
1
-
6
8
8
-
-
1
28
-
15
-
-
2
8
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
10
3
1
-
1
-
6
-
8
-
8
-
-
1
28
-
-
-
2
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
288997
Maley
Probing the infra-structure of ...
Enterobacteria phage T2, Gallus gallus
Adv. Enzyme Regul.
22
413-430
1984
3
-
-
1
-
-
-
2
-
-
-
8
-
5
-
-
1
2
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
2
-
-
-
8
-
-
-
1
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289000
McIntosh
Isolation of a Saccharomyces c ...
no activity in Escherichia coli, no activity in Salmonella typhimurium, Saccharomyces cerevisiae
J. Bacteriol.
158
644-649
1984
1
-
-
-
-
-
-
2
-
1
-
1
-
5
-
-
1
-
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
1
-
-
-
-
1
1
2
-
-
-
1
-
3
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289002
Mastrantonio
Analysis of competition for su ...
Equus asinus
Eur. J. Biochem.
137
421-427
1983
2
-
-
-
-
-
3
3
-
-
1
1
-
2
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289003
Raia
Reversal of the effect of the ...
Equus asinus
J. Mol. Biol.
157
557-570
1982
5
-
-
-
-
1
8
2
-
-
1
1
-
1
-
-
1
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
8
-
2
-
-
1
1
-
-
-
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288999
Ellims
Deoxycytidylate deaminase. Pur ...
Homo sapiens
J. Biol. Chem.
256
6335-6340
1981
7
-
-
-
-
3
16
1
-
4
2
2
-
2
-
-
1
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
7
-
-
-
-
-
3
-
16
-
1
-
4
2
2
-
-
-
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
288998
Nucci
Freezing of dCMP aminohydrolas ...
Equus asinus
J. Mol. Biol.
124
133-145
1978
2
-
-
-
-
-
3
1
-
1
2
1
-
1
-
-
1
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289004
Maley
Deoxycytidylate deaminase from ...
Enterobacteria phage T2, no activity in Escherichia coli
Methods Enzymol.
51
412-418
1978
2
-
-
-
-
3
1
4
-
4
1
2
-
3
-
-
1
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
3
-
1
-
4
-
4
1
2
-
-
-
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
289005
Mollgaard
Deoxycytidylate deaminase from ...
Bacillusphage phiE, Bacillus subtilis, Bacillus subtilis ED40, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium
J. Biol. Chem.
253
3536-3542
1978
9
-
-
-
-
4
2
2
-
10
1
2
-
7
-
-
1
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
4
-
2
-
2
-
10
1
2
-
-
-
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289006
Dosseva.I.M.; Tomov
-
Stabilizing effect of ethylene ...
Mus musculus
Dokl. Bolg. Akad. Nauk
28
241-244
1975
-
-
-
-
-
5
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
289007
Rolton
Deoxycytidylate deaminase. Evi ...
Cricetinae, Herpes simplex virus
Biochem. J.
143
403-409
1974
6
-
-
-
-
3
6
4
-
2
-
2
-
2
-
-
2
-
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
6
-
-
-
-
-
3
-
6
-
4
-
2
-
2
-
-
-
2
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
289008
Rolton
Deoxycytidylate deaminase. Pro ...
Cricetinae
Biochem. J.
141
211-217
1974
1
-
-
-
-
3
2
1
-
3
2
1
-
1
-
-
1
1
-
2
1
2
4
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
1
-
-
3
-
2
-
1
-
3
2
1
-
-
-
1
-
2
1
2
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289009
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Escherichia virus T4, Gallus gallus
J. Biol. Chem.
247
931-939
1972
5
-
-
-
-
2
3
4
-
-
1
3
-
3
-
-
2
3
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
5
-
-
-
-
-
2
-
3
-
4
-
-
1
3
-
-
-
2
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
-
-
-
289010
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Gallus gallus
J. Biol. Chem.
247
940-945
1972
3
-
-
-
-
2
3
-
-
-
2
2
-
2
-
-
-
2
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
3
-
-
-
-
2
2
-
-
-
-
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289011
Sergott
On the regulation of a bacteri ...
Lactobacillus acidophilus, Staphylococcus aureus
J. Biol. Chem.
246
7755-7758
1971
4
-
-
-
-
1
6
-
-
-
-
2
-
2
-
-
2
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
-
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-