BRENDA - Enzyme Database
show all sequences of 3.5.4.12

Properties of an affinity-column-purified human deoxycytidylate deaminase

Maley, G.F.; Lobo, A.P.; Maley, F.; Biochim. Biophys. Acta 1162, 161-170 (1993)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dCTP
activation by dCTP is reversed by addition of dTTP; allosteric activator; positive allosteric effector
Homo sapiens
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Homo sapiens
Cloned(Commentary)
Commentary
Organism
cDNA from HeLa cells can be expressed in an active form
Homo sapiens
General Stability
General Stability
Organism
2-mercaptoethanol stabilizes
Homo sapiens
dCTP and MgCl2 stabilize
Homo sapiens
enzyme extremely labile, stabilized by dCTP /Mg2+
Homo sapiens
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
Homo sapiens
ethyleneglycol, 10-20% stabilizes
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
competitive inhibition, Ki: 0.000012 mM
Homo sapiens
3,4,5,6-tetrahydro-2'-dUMP
potent inhibitor, Ki: 0.00001 mM
Homo sapiens
5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
Homo sapiens
5-Hg-dCMP
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
Homo sapiens
5-Hg-dUMP
potent inhibitor, 0.0001 mM: 54% inhibition
Homo sapiens
dAMP
-
Homo sapiens
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Homo sapiens
dGMP
-
Homo sapiens
dTTP
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
Homo sapiens
dUMP
-
Homo sapiens
Hg(CH3COO)2
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Co2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
divalent cations
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Mg2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Homo sapiens
Mn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
additional information
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Zn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE
Homo sapiens
117000
-
in the presence of dCTP and Mg2+, 4°C, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Homo sapiens
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
dUMP + NH3
-
Homo sapiens
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
human
-
Purification (Commentary)
Commentary
Organism
HeLa cells
Homo sapiens
Reaction
Reaction
Commentary
Organism
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; most dramatic allosteric response at substrate levels 0.1 mM dCMP or less; regulation, allosteric end-product regulation
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
481
-
-
Homo sapiens
Storage Stability
Storage Stability
Organism
-20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-Hg-dCMP + H2O
very poor substrate in the absence of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
CMP + H2O
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
289018
Homo sapiens
UMP + NH3
-
-
-
-
dCMP + H2O
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
dCMP + H2O
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
additional information
substrate specificity
289018
Homo sapiens
?
-
-
-
-
additional information
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
289018
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE; 6 identical subunits
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
optimum condition
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
960
-
dCMP
-
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
HeLa cells, in presence or absence of dCTP and Mg2+
Homo sapiens
8
-
assay at
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dCTP
activation by dCTP is reversed by addition of dTTP; allosteric activator; positive allosteric effector
Homo sapiens
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA from HeLa cells can be expressed in an active form
Homo sapiens
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol stabilizes
Homo sapiens
dCTP and MgCl2 stabilize
Homo sapiens
enzyme extremely labile, stabilized by dCTP /Mg2+
Homo sapiens
ethyleneglycol prevents protein denaturation during freezing in the presence of 2-mercaptoethanol
Homo sapiens
ethyleneglycol, 10-20% stabilizes
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-beta-D-deoxyribose-pyrimidin-2-one 5'-phosphate
competitive inhibition, Ki: 0.000012 mM
Homo sapiens
3,4,5,6-tetrahydro-2'-dUMP
potent inhibitor, Ki: 0.00001 mM
Homo sapiens
5-(acryloylamino-pentanol-1)2'-beta-D-dUMP
-
Homo sapiens
5-Hg-dCMP
potent inhibitor, in the absence of 2-mercaptoethanol, 0.00024 mM: 37% inhibition, 0.0024 mM: 70% inhibition
Homo sapiens
5-Hg-dUMP
potent inhibitor, 0.0001 mM: 54% inhibition
Homo sapiens
dAMP
-
Homo sapiens
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Homo sapiens
dGMP
-
Homo sapiens
dTTP
0.0015 mM: 50% inhibition. dTTP inhibition reversed by dCTP; allosteric inhibitor
Homo sapiens
dUMP
-
Homo sapiens
Hg(CH3COO)2
Hg(acetate)2; mercuroacetate, potent inhibitor, 0.0002 mM: 26% inhibition, 0.001 mM: 40% inhibition
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Co2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
divalent cations
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Mg2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective; required for activation by dCTP and for inhibition by dTTP; required for activation by dCTP, Mg2+ in the absence of dCTP: no effect
Homo sapiens
Mn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
additional information
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Zn2+
divalent cations not necessary to improve activity, activation by dCTP and inhibition by dTTP both show an absolute requirement for a divalent cation. Mg2+, Ca2+ and Mn2+ almost equally effective in promoting activation by dCTP, Cu2+, Zn2+, and Co2+ are ineffective
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20000
-
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE
Homo sapiens
117000
-
in the presence of dCTP and Mg2+, 4°C, gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Homo sapiens
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
dUMP + NH3
-
Homo sapiens
-
Purification (Commentary) (protein specific)
Commentary
Organism
HeLa cells
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HeLa cell
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
481
-
-
Homo sapiens
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10-20% ethyleneglycol, 0.01 mM dCTP, 1 mM MgCl2, 20 mM 2-mercaptoethanol and 0.05% Triton X-100, 1 year, no loss in activity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-Hg-dCMP + H2O
very poor substrate in the absence of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289018
Homo sapiens
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
HeLa cells, good substrate
289018
Homo sapiens
5-methyl-dUMP + NH3
-
-
-
?
CMP + H2O
HeLa cells, at 1 mM, in presence and absence of dCTP and Mg2+: not a substrate
289018
Homo sapiens
UMP + NH3
-
-
-
-
dCMP + H2O
most dramatic allosteric response at substrate levels 0.1 mM dCMP or less
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
dCMP + H2O
enzyme contributes formation of DNA by providing dUMP as substrate for thymidylate synthase. Potential role in cell division
289018
Homo sapiens
dUMP + NH3
-
289018
Homo sapiens
-
additional information
substrate specificity
289018
Homo sapiens
?
-
-
-
-
additional information
HeLa cells, no activity with CDP at 1 mM, in presence and absence of dCTP and Mg2+
289018
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 20000, HeLa cells, SDS-PAGE and amino acid sequence; 6 * 20000, SDS-PAGE; 6 identical subunits
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
optimum condition
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
960
-
dCMP
-
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
HeLa cells, in presence or absence of dCTP and Mg2+
Homo sapiens
8
-
assay at
Homo sapiens
Other publictions for EC 3.5.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734322
Marx
The first crystal structure of ...
Cyanophage S-TIM5
J. Biol. Chem.
290
682-690
2015
-
-
1
1
10
-
1
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
1
10
-
-
1
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
734401
Xu
STRIPE2 encodes a putative dCM ...
Oryza sativa
J. Genet. Genomics
41
539-548
2014
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712083
Jansen
Deoxyuridine analog nucleotide ...
Homo sapiens
Fundam. Clin. Pharmacol.
25
172-185
2011
1
-
-
-
-
-
8
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
711628
Li
DNA mismatch repair (MMR)-depe ...
Homo sapiens
Br. J. Pharmacol.
158
679-692
2009
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688405
Hou
Crystal structures of Streptoc ...
Streptococcus mutans
J. Mol. Biol.
377
220-231
2008
1
-
1
1
-
-
1
-
-
5
-
1
-
2
-
-
1
-
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
5
-
1
-
-
-
1
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686248
Liskay
Involvement of deoxycytidylate ...
Saccharomyces cerevisiae
Curr. Biol.
17
R755-R757
2007
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
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1
-
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2
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688732
Zhang
Chloroviruses encode a bifunct ...
Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
2007
1
-
1
-
-
-
3
2
-
5
1
2
-
4
-
-
1
-
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
2
-
5
1
2
-
-
-
1
-
-
-
1
4
1
1
1
-
2
2
1
-
-
-
-
-
-
-
-
667587
Almog
Three-dimensional structure of ...
Escherichia virus T4
Biochemistry
43
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2004
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1
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669680
Gribaudo
Human cytomegalovirus requires ...
Homo sapiens
J. Gen. Virol.
84
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2003
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1
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3
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670328
Liou
Assessment of the effect of ph ...
Homo sapiens
Mol. Pharmacol.
63
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2003
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10
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4
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1
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6
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10
6
1
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1
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1
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1
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1
-
-
4
-
-
-
-
-
1
-
-
-
-
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-
-
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-
667541
Cha
Modifications of deoxycytidine ...
Rattus norvegicus
Biochem. Pharmacol.
63
717-723
2002
1
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-
-
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1
4
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1
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1
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1
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1
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4
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1
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3
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668584
Jost
5-Methyldeoxycytidine monophos ...
Homo sapiens
FEBS Lett.
519
128-134
2002
1
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-
-
-
-
1
1
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1
-
3
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2
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1
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2
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5
-
1
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1
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1
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-
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-
-
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1
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1
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1
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3
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1
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289015
Keefe
A T4-phage deoxycytidylate dea ...
Enterobacteria phage T2, Enterobacteria phage T6, Escherichia virus T4
J. Biol. Chem.
275
12598-12602
2000
7
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1
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3
4
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5
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2
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4
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1
3
1
-
4
2
4
5
1
-
-
5
2
-
-
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7
-
1
-
-
3
4
-
4
-
5
-
4
9
2
-
-
-
1
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
-
-
-
289014
Nucci
Interaction of the high-affini ...
Equus asinus
Arch. Biochem. Biophys.
310
49-53
1994
2
1
-
-
-
-
3
1
-
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1
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1
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-
1
1
-
1
-
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1
-
-
-
-
-
-
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-
-
-
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2
1
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-
-
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3
-
1
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1
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-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289017
Moore
Identification of a site neces ...
Escherichia virus T4, Homo sapiens
Biochemistry
33
2104-2112
1994
3
-
2
-
1
-
6
-
-
1
-
2
-
2
-
-
1
2
-
-
-
-
4
2
2
-
-
-
-
-
-
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-
-
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3
-
2
-
-
1
-
-
6
-
-
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1
-
2
-
-
-
1
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
289018
Maley
Properties of an affinity-colu ...
Homo sapiens
Biochim. Biophys. Acta
1162
161-170
1993
2
-
1
-
-
5
11
-
-
7
2
1
-
3
-
-
1
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
2
-
1
-
-
-
5
-
11
-
-
-
7
2
1
-
-
-
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
289016
Xu
Modulation of deoxycytidylate ...
Homo sapiens
Biochem. Pharmacol.
44
1819-1827
1992
1
1
-
-
-
-
7
-
-
-
-
2
-
2
-
-
1
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
-
-
-
2
-
-
-
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289012
Nucci
Allosteric modifier and substr ...
Equus asinus
Arch. Biochem. Biophys.
289
19-25
1991
2
-
-
-
-
1
4
1
-
-
2
1
-
3
-
-
1
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
289013
Whitehead
Hill coefficient ratios give b ...
Equus asinus
Arch. Biochem. Biophys.
289
12-18
1991
2
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289019
Riva
-
Correlation between deoxycytid ...
Homo sapiens
J. Cell. Pharmacol.
1
79-85
1990
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
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1
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-
-
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289020
Maley
A tale of two enzymes, deoxycy ...
Bacillus subtilis, Enterobacteria phage T2, Enterobacteria phage T6, Equus asinus, Escherichia virus T4, Gallus gallus, Herpes simplex virus, Homo sapiens, Macaca mulatta polyomavirus 1, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Saccharomyces cerevisiae, Sea urchin
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
10
3
1
1
-
6
8
8
-
-
1
28
-
15
-
-
2
8
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
10
3
1
-
1
-
6
-
8
-
8
-
-
1
28
-
-
-
2
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
288997
Maley
Probing the infra-structure of ...
Enterobacteria phage T2, Gallus gallus
Adv. Enzyme Regul.
22
413-430
1984
3
-
-
1
-
-
-
2
-
-
-
8
-
5
-
-
1
2
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
2
-
-
-
8
-
-
-
1
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289000
McIntosh
Isolation of a Saccharomyces c ...
no activity in Escherichia coli, no activity in Salmonella typhimurium, Saccharomyces cerevisiae
J. Bacteriol.
158
644-649
1984
1
-
-
-
-
-
-
2
-
1
-
1
-
5
-
-
1
-
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
1
-
-
-
-
1
1
2
-
-
-
1
-
3
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289002
Mastrantonio
Analysis of competition for su ...
Equus asinus
Eur. J. Biochem.
137
421-427
1983
2
-
-
-
-
-
3
3
-
-
1
1
-
2
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289003
Raia
Reversal of the effect of the ...
Equus asinus
J. Mol. Biol.
157
557-570
1982
5
-
-
-
-
1
8
2
-
-
1
1
-
1
-
-
1
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
8
-
2
-
-
1
1
-
-
-
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288999
Ellims
Deoxycytidylate deaminase. Pur ...
Homo sapiens
J. Biol. Chem.
256
6335-6340
1981
7
-
-
-
-
3
16
1
-
4
2
2
-
2
-
-
1
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
7
-
-
-
-
-
3
-
16
-
1
-
4
2
2
-
-
-
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
288998
Nucci
Freezing of dCMP aminohydrolas ...
Equus asinus
J. Mol. Biol.
124
133-145
1978
2
-
-
-
-
-
3
1
-
1
2
1
-
1
-
-
1
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289004
Maley
Deoxycytidylate deaminase from ...
Enterobacteria phage T2, no activity in Escherichia coli
Methods Enzymol.
51
412-418
1978
2
-
-
-
-
3
1
4
-
4
1
2
-
3
-
-
1
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
3
-
1
-
4
-
4
1
2
-
-
-
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
289005
Mollgaard
Deoxycytidylate deaminase from ...
Bacillusphage phiE, Bacillus subtilis, Bacillus subtilis ED40, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium
J. Biol. Chem.
253
3536-3542
1978
9
-
-
-
-
4
2
2
-
10
1
2
-
7
-
-
1
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
4
-
2
-
2
-
10
1
2
-
-
-
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289006
Dosseva.I.M.; Tomov
-
Stabilizing effect of ethylene ...
Mus musculus
Dokl. Bolg. Akad. Nauk
28
241-244
1975
-
-
-
-
-
5
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
289007
Rolton
Deoxycytidylate deaminase. Evi ...
Cricetinae, Herpes simplex virus
Biochem. J.
143
403-409
1974
6
-
-
-
-
3
6
4
-
2
-
2
-
2
-
-
2
-
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
6
-
-
-
-
-
3
-
6
-
4
-
2
-
2
-
-
-
2
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
289008
Rolton
Deoxycytidylate deaminase. Pro ...
Cricetinae
Biochem. J.
141
211-217
1974
1
-
-
-
-
3
2
1
-
3
2
1
-
1
-
-
1
1
-
2
1
2
4
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
1
-
-
3
-
2
-
1
-
3
2
1
-
-
-
1
-
2
1
2
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289009
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Escherichia virus T4, Gallus gallus
J. Biol. Chem.
247
931-939
1972
5
-
-
-
-
2
3
4
-
-
1
3
-
3
-
-
2
3
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
5
-
-
-
-
-
2
-
3
-
4
-
-
1
3
-
-
-
2
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
-
-
-
289010
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Gallus gallus
J. Biol. Chem.
247
940-945
1972
3
-
-
-
-
2
3
-
-
-
2
2
-
2
-
-
-
2
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
3
-
-
-
-
2
2
-
-
-
-
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289011
Sergott
On the regulation of a bacteri ...
Lactobacillus acidophilus, Staphylococcus aureus
J. Biol. Chem.
246
7755-7758
1971
4
-
-
-
-
1
6
-
-
-
-
2
-
2
-
-
2
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
-
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-