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Literature summary for 3.5.4.12 extracted from

  • Maley, G.F.; MacColl, R.; Maley, F.
    T2r+ bacteriophage-induced enzymes. II. The subunit structure of deoxycytidylate deaminase (1972), J. Biol. Chem., 247, 940-945.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
5-hydroxymethyl-dCTP natural positive allosteric effector, enzyme is much more effectively regulated by its natural effector, 5-hydroxymethyl-dCTP, than by dCTP, binding of 5-hydroxymethyl-dCTP is much more pH dependent than dCTP Escherichia phage T2
5-hydroxymethyl-dCTP pH-dependent activation Escherichia phage T2
dCTP pH-dependent activation Gallus gallus
dCTP positive allosteric effector Gallus gallus
dCTP positive allosteric effector Escherichia phage T2
dCTP activation by dCTP decreases markedly as the pH is increased, pH-dependent activation Escherichia phage T2

General Stability

General Stability Organism
2-mercaptoethanol stabilizes Escherichia phage T2
guanidine hydrochloride, 1 M, complete loss in activity accompanied by disaggregation of enzyme, 6 M: denaturation, complete reactivation with 50 mM 2-mercaptoethanol Escherichia phage T2

Inhibitors

Inhibitors Comment Organism Structure
dTTP allosteric inhibitor Escherichia phage T2
dTTP allosteric inhibitor Gallus gallus
guanidine hydrochloride 0.5 M: 50% inhibition, 1 M: 100% inhibition Escherichia phage T2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20200
-
6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE Escherichia phage T2
124000
-
PAGE and sedimentation equilibrium Escherichia phage T2

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Gallus gallus
-
dUMP + NH3
-
?
dCMP + H2O Escherichia phage T2
-
dUMP + NH3
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage T2
-
T2r+-infected Escherichia coli B
-
Gallus gallus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Gallus gallus
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Escherichia phage T2
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Gallus gallus
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Escherichia phage T2

Renatured (Commentary)

Renatured (Comment) Organism
after treatment with guanidine hydrochloride, 6 M, complete reactivation with 50 mM 2-mercaptoethanol, original hexameric structure restored Escherichia phage T2

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCMP + H2O
-
Gallus gallus dUMP + NH3
-
?
dCMP + H2O
-
Escherichia phage T2 dUMP + NH3
-
?

Subunits

Subunits Comment Organism
hexamer 6 identical subunits Gallus gallus
hexamer 6 identical subunits Escherichia phage T2
hexamer hexamer is the active form Escherichia phage T2
hexamer 6 * 20200, sedimentation in 6 M guanidine hydrochloride containing 0.1 M 2-mercaptoethanol and SDS-PAGE Escherichia phage T2