BRENDA - Enzyme Database
show all sequences of 3.5.4.12

Deoxycytidylate deaminase from Bacillus subtilis. Purification, characterization, and physiological function

Mollgaard, H.; Neuhard, J.; J. Biol. Chem. 253, 3536-3542 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
CTP
no activation
Bacillus subtilis
dCDP
100% activation
Bacillus subtilis
dCTP
dCTP not required and not activared by dCTP
Bacillusphage phiE
dCTP
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
dCTP
dCTP not required and not activared by dCTP
bacteriophage SP8
dTTP
no activation
Bacillus subtilis
dUTP
89% activation
Bacillus subtilis
additional information
no activation by ATP, UTP, CTP and dTTP
Bacillus subtilis
General Stability
General Stability
Organism
2-mercaptoethanol stabilizes
Bacillus subtilis
thiols stabilize
Bacillus subtilis
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
Bacillus subtilis
Zn2+ or dCTP stabilize, protect against inactivation
Bacillus subtilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
dTTP
no inhibition by dTTP
bacteriophage SP8
EDTA
completely
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
0.14
-
dCMP
-
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
no activation
Bacillus subtilis
Co2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
metal ion
required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
Mg2+
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+; no activation
Bacillus subtilis
Mn2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
additional information
metal ions not required
Bacillusphage phiE
additional information
metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
additional information
metal ions not required
bacteriophage SP8
Ni2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
Zn2+
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
gel filtration and sucrose density gradient centrifugation
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis
?
dCMP + H2O
Bacillus subtilis ED40
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis ED40
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis ED40
-
-
-
Bacillusphage phiE
-
host Bacillus subtilis
-
bacteriophage SP8
-
host Bacillus subtilis
-
no activity in Escherichia coli
-
wild type Escherichia coli contains no dCMP deaminase
-
no activity in Salmonella typhimurium
-
do not contain dCMP deaminase
-
Purification (Commentary)
Commentary
Organism
partial
Bacillus subtilis
Reaction
Reaction
Commentary
Organism
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme; regulation, allosteric end-product regulation
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.36
-
-
Bacillus subtilis
Storage Stability
Storage Stability
Organism
-70C, pH 7.8, 2 mM 2-mercaptoethanol
Bacillus subtilis
0C, 2-mercaptoethanol, stable for more than 6 h
Bacillus subtilis
0C, half-life: 30 min
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis
UMP + NH3
-
-
-
?
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis ED40
UMP + NH3
-
-
-
?
dCMP + H2O
-
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
dCMP + H2O
-
289005
Bacillusphage phiE
dUMP + NH3
-
289005
Bacillusphage phiE
?
dCMP + H2O
-
289005
bacteriophage SP8
dUMP + NH3
-
289005
bacteriophage SP8
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
dCMP + H2O
-
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
additional information
substrate specificity
289005
Bacillus subtilis
?
-
-
-
-
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
289005
Bacillus subtilis ED40
?
-
-
-
-
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis ED40
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-
-
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation
Bacillus subtilis
50
-
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50C
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
pH optimum around
Bacillus subtilis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
50% maximal activity at pH 7.0 and 8.5
Bacillus subtilis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
CTP
no activation
Bacillus subtilis
dCDP
100% activation
Bacillus subtilis
dCTP
dCTP not required and not activared by dCTP
Bacillusphage phiE
dCTP
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol
Bacillus subtilis
dCTP
dCTP not required and not activared by dCTP
bacteriophage SP8
dTTP
no activation
Bacillus subtilis
dUTP
89% activation
Bacillus subtilis
additional information
no activation by ATP, UTP, CTP and dTTP
Bacillus subtilis
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol stabilizes
Bacillus subtilis
thiols stabilize
Bacillus subtilis
thiols, such as 2-mercaptoethanol or dithiothreitol, stabilize, in the absence of thiols the enzyme is highly unstable
Bacillus subtilis
Zn2+ or dCTP stabilize, protect against inactivation
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dTTP
no inhibition by dTTP
bacteriophage SP8
EDTA
completely
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Bacillus subtilis
0.14
-
dCMP
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
no activation
Bacillus subtilis
Co2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
metal ion
required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
Mg2+
enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+; no activation
Bacillus subtilis
Mn2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
additional information
metal ions not required
Bacillusphage phiE
additional information
metal ion required, in the absence of metal ions: 40% activity, Zn2+ highest activity, around 20% activity with Co2+, Mn2+, and Ni2+, no activity in the presence of Mg2+, Ca2+ and Fe2+
Bacillus subtilis
additional information
metal ions not required
bacteriophage SP8
Ni2+
metal ion required, highest activity with Zn2+, about 20% activity with Co2+, Mn2+, and Ni2+
Bacillus subtilis
Zn2+
required, enzyme requires dCTP, Zn2+, and 2-mercaptoethanol, Mg2+ cannot substitute for Zn2+
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
170000
-
gel filtration and sucrose density gradient centrifugation
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Bacillus subtilis
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis
?
dCMP + H2O
Bacillus subtilis ED40
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
dUMP + NH3
-
Bacillus subtilis ED40
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
6.36
-
-
Bacillus subtilis
Storage Stability (protein specific)
Storage Stability
Organism
-70C, pH 7.8, 2 mM 2-mercaptoethanol
Bacillus subtilis
0C, 2-mercaptoethanol, stable for more than 6 h
Bacillus subtilis
0C, half-life: 30 min
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis
UMP + NH3
-
-
-
?
CMP + H2O
slight activity, about 5% compared with dCMP
289005
Bacillus subtilis ED40
UMP + NH3
-
-
-
?
dCMP + H2O
-
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
dCMP + H2O
-
289005
Bacillusphage phiE
dUMP + NH3
-
289005
Bacillusphage phiE
?
dCMP + H2O
-
289005
bacteriophage SP8
dUMP + NH3
-
289005
bacteriophage SP8
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis
dUMP + NH3
-
289005
Bacillus subtilis
?
dCMP + H2O
-
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
dCMP + H2O
importance in the generation of dUMP, precursor for synthesis of thymidine nucleotides
289005
Bacillus subtilis ED40
dUMP + NH3
-
289005
Bacillus subtilis ED40
?
additional information
substrate specificity
289005
Bacillus subtilis
?
-
-
-
-
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis
?
-
-
-
-
additional information
substrate specificity
289005
Bacillus subtilis ED40
?
-
-
-
-
additional information
no activity with dCDP, dCTP, CDP, CTP
289005
Bacillus subtilis ED40
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Bacillus subtilis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-
-
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation
Bacillus subtilis
50
-
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50C
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
pH optimum around
Bacillus subtilis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
8.5
50% maximal activity at pH 7.0 and 8.5
Bacillus subtilis
Other publictions for EC 3.5.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734322
Marx
The first crystal structure of ...
Cyanophage S-TIM5
J. Biol. Chem.
290
682-690
2015
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-
1
1
10
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1
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1
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1
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1
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1
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2
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1
1
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1
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1
10
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1
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1
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1
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1
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2
-
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1
1
-
-
-
-
-
-
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-
734401
Xu
STRIPE2 encodes a putative dCM ...
Oryza sativa
J. Genet. Genomics
41
539-548
2014
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-
1
-
-
-
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-
2
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1
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2
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1
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1
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712083
Jansen
Deoxyuridine analog nucleotide ...
Homo sapiens
Fundam. Clin. Pharmacol.
25
172-185
2011
1
-
-
-
-
-
8
-
-
-
-
1
-
1
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7
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1
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8
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7
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1
1
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-
711628
Li
DNA mismatch repair (MMR)-depe ...
Homo sapiens
Br. J. Pharmacol.
158
679-692
2009
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-
-
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1
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1
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1
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1
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688405
Hou
Crystal structures of Streptoc ...
Streptococcus mutans
J. Mol. Biol.
377
220-231
2008
1
-
1
1
-
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1
-
-
5
-
1
-
2
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1
-
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2
2
1
-
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1
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1
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1
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2
2
1
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1
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-
686248
Liskay
Involvement of deoxycytidylate ...
Saccharomyces cerevisiae
Curr. Biol.
17
R755-R757
2007
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2
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1
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2
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688732
Zhang
Chloroviruses encode a bifunct ...
Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
2007
1
-
1
-
-
-
3
2
-
5
1
2
-
4
-
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1
-
-
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1
4
1
1
1
-
2
2
1
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1
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1
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3
-
2
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5
1
2
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1
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1
4
1
1
1
-
2
2
1
-
-
-
-
-
-
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-
667587
Almog
Three-dimensional structure of ...
Escherichia virus T4
Biochemistry
43
13715-13723
2004
1
1
1
1
1
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2
-
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1
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1
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1
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1
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2
1
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1
1
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1
1
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2
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1
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1
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2
1
-
-
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-
669680
Gribaudo
Human cytomegalovirus requires ...
Homo sapiens
J. Gen. Virol.
84
1437-1441
2003
1
-
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2
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3
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1
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3
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1
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3
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670328
Liou
Assessment of the effect of ph ...
Homo sapiens
Mol. Pharmacol.
63
105-110
2003
-
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10
1
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1
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1
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3
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1
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1
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4
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1
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6
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10
6
1
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1
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1
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1
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1
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4
-
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1
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667541
Cha
Modifications of deoxycytidine ...
Rattus norvegicus
Biochem. Pharmacol.
63
717-723
2002
1
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-
-
-
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1
4
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1
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1
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3
1
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2
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1
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1
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1
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1
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4
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1
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3
1
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2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668584
Jost
5-Methyldeoxycytidine monophos ...
Homo sapiens
FEBS Lett.
519
128-134
2002
1
-
-
-
-
-
1
1
-
1
-
3
-
2
-
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1
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2
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5
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1
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1
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1
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1
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1
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1
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3
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1
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289015
Keefe
A T4-phage deoxycytidylate dea ...
Enterobacteria phage T2, Enterobacteria phage T6, Escherichia virus T4
J. Biol. Chem.
275
12598-12602
2000
7
-
1
-
3
4
4
5
-
4
9
2
-
4
-
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1
3
1
-
4
2
4
5
1
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-
5
2
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7
-
1
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3
4
-
4
-
5
-
4
9
2
-
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1
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
-
-
-
289014
Nucci
Interaction of the high-affini ...
Equus asinus
Arch. Biochem. Biophys.
310
49-53
1994
2
1
-
-
-
-
3
1
-
-
-
1
-
1
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1
1
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1
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1
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2
1
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3
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1
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1
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1
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289017
Moore
Identification of a site neces ...
Escherichia virus T4, Homo sapiens
Biochemistry
33
2104-2112
1994
3
-
2
-
1
-
6
-
-
1
-
2
-
2
-
-
1
2
-
-
-
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4
2
2
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3
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2
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1
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6
-
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1
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2
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1
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
289018
Maley
Properties of an affinity-colu ...
Homo sapiens
Biochim. Biophys. Acta
1162
161-170
1993
2
-
1
-
-
5
11
-
-
7
2
1
-
3
-
-
1
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
2
-
1
-
-
-
5
-
11
-
-
-
7
2
1
-
-
-
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
289016
Xu
Modulation of deoxycytidylate ...
Homo sapiens
Biochem. Pharmacol.
44
1819-1827
1992
1
1
-
-
-
-
7
-
-
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2
-
2
-
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1
1
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3
-
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4
-
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-
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1
1
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7
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2
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1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289012
Nucci
Allosteric modifier and substr ...
Equus asinus
Arch. Biochem. Biophys.
289
19-25
1991
2
-
-
-
-
1
4
1
-
-
2
1
-
3
-
-
1
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
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2
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
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1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
289013
Whitehead
Hill coefficient ratios give b ...
Equus asinus
Arch. Biochem. Biophys.
289
12-18
1991
2
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289019
Riva
-
Correlation between deoxycytid ...
Homo sapiens
J. Cell. Pharmacol.
1
79-85
1990
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
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1
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-
-
-
-
-
-
-
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-
-
-
-
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1
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1
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2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289020
Maley
A tale of two enzymes, deoxycy ...
Bacillus subtilis, Enterobacteria phage T2, Enterobacteria phage T6, Equus asinus, Escherichia virus T4, Gallus gallus, Herpes simplex virus, Homo sapiens, Macaca mulatta polyomavirus 1, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Saccharomyces cerevisiae, Sea urchin
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
10
3
1
1
-
6
8
8
-
-
1
28
-
15
-
-
2
8
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
10
3
1
-
1
-
6
-
8
-
8
-
-
1
28
-
-
-
2
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
288997
Maley
Probing the infra-structure of ...
Enterobacteria phage T2, Gallus gallus
Adv. Enzyme Regul.
22
413-430
1984
3
-
-
1
-
-
-
2
-
-
-
8
-
5
-
-
1
2
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
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3
-
-
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1
-
-
-
-
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2
-
-
-
8
-
-
-
1
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289000
McIntosh
Isolation of a Saccharomyces c ...
no activity in Escherichia coli, no activity in Salmonella typhimurium, Saccharomyces cerevisiae
J. Bacteriol.
158
644-649
1984
1
-
-
-
-
-
-
2
-
1
-
1
-
5
-
-
1
-
-
-
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1
9
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
1
-
-
-
-
1
1
2
-
-
-
1
-
3
-
-
-
1
-
-
-
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2
-
1
-
-
-
1
-
-
-
-
-
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1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289002
Mastrantonio
Analysis of competition for su ...
Equus asinus
Eur. J. Biochem.
137
421-427
1983
2
-
-
-
-
-
3
3
-
-
1
1
-
2
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289003
Raia
Reversal of the effect of the ...
Equus asinus
J. Mol. Biol.
157
557-570
1982
5
-
-
-
-
1
8
2
-
-
1
1
-
1
-
-
1
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
8
-
2
-
-
1
1
-
-
-
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288999
Ellims
Deoxycytidylate deaminase. Pur ...
Homo sapiens
J. Biol. Chem.
256
6335-6340
1981
7
-
-
-
-
3
16
1
-
4
2
2
-
2
-
-
1
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
7
-
-
-
-
-
3
-
16
-
1
-
4
2
2
-
-
-
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
288998
Nucci
Freezing of dCMP aminohydrolas ...
Equus asinus
J. Mol. Biol.
124
133-145
1978
2
-
-
-
-
-
3
1
-
1
2
1
-
1
-
-
1
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289004
Maley
Deoxycytidylate deaminase from ...
Enterobacteria phage T2, no activity in Escherichia coli
Methods Enzymol.
51
412-418
1978
2
-
-
-
-
3
1
4
-
4
1
2
-
3
-
-
1
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
3
-
1
-
4
-
4
1
2
-
-
-
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
289005
Mollgaard
Deoxycytidylate deaminase from ...
Bacillusphage phiE, Bacillus subtilis, Bacillus subtilis ED40, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium
J. Biol. Chem.
253
3536-3542
1978
9
-
-
-
-
4
2
2
-
10
1
2
-
7
-
-
1
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
4
-
2
-
2
-
10
1
2
-
-
-
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289006
Dosseva.I.M.; Tomov
-
Stabilizing effect of ethylene ...
Mus musculus
Dokl. Bolg. Akad. Nauk
28
241-244
1975
-
-
-
-
-
5
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
289007
Rolton
Deoxycytidylate deaminase. Evi ...
Cricetinae, Herpes simplex virus
Biochem. J.
143
403-409
1974
6
-
-
-
-
3
6
4
-
2
-
2
-
2
-
-
2
-
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
6
-
-
-
-
-
3
-
6
-
4
-
2
-
2
-
-
-
2
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
289008
Rolton
Deoxycytidylate deaminase. Pro ...
Cricetinae
Biochem. J.
141
211-217
1974
1
-
-
-
-
3
2
1
-
3
2
1
-
1
-
-
1
1
-
2
1
2
4
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
1
-
-
3
-
2
-
1
-
3
2
1
-
-
-
1
-
2
1
2
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289009
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Escherichia virus T4, Gallus gallus
J. Biol. Chem.
247
931-939
1972
5
-
-
-
-
2
3
4
-
-
1
3
-
3
-
-
2
3
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
5
-
-
-
-
-
2
-
3
-
4
-
-
1
3
-
-
-
2
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
-
-
-
289010
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Gallus gallus
J. Biol. Chem.
247
940-945
1972
3
-
-
-
-
2
3
-
-
-
2
2
-
2
-
-
-
2
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
3
-
-
-
-
2
2
-
-
-
-
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289011
Sergott
On the regulation of a bacteri ...
Lactobacillus acidophilus, Staphylococcus aureus
J. Biol. Chem.
246
7755-7758
1971
4
-
-
-
-
1
6
-
-
-
-
2
-
2
-
-
2
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
-
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-