BRENDA - Enzyme Database
show all sequences of 3.5.4.12

Reversal of the effect of the allosteric ligands of dCMP-aminohydrolase and stabilization of the enzyme in the T form

Raia, C.A.; Nucci, R.; Vaccaro, C.; Sepe, S.; Rella, R.; J. Mol. Biol. 157, 557-570 (1982)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ammonium sulfate
substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP
Equus asinus
dCTP
allosteric activator; allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Equus asinus
Glutaraldehyde
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition
Equus asinus
General Stability
General Stability
Organism
dCTP or glycerol stabilize
Equus asinus
Inhibitors
Inhibitors
Commentary
Organism
Structure
5-Hg-dCMP
irreversible competitive inhibitor, in the absence of mercaptoethanol
Equus asinus
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dGMP
competitive inhibitor
Equus asinus
dTTP
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dUMP
competitive inhibitor
Equus asinus
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
Equus asinus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic experiments; kinetic properties and parameters
Equus asinus
0.26
-
dCMP-Hg-S-CH2-CH2-OH
-
Equus asinus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Equus asinus
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
dUMP + NH3
-
Equus asinus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Equus asinus
-
donkey; three conformational isomers of dCMPase, the conformation of the enzyme with no ligands, the activated form, R form: dCTP-Mg complex and the inhibited form, T form: dTTP-Mg complex
-
Purification (Commentary)
Commentary
Organism
-
Equus asinus
Reaction
Reaction
Commentary
Organism
dCMP + H2O = dUMP + NH3
highly regulated allosteric enzyme
Equus asinus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Equus asinus
23
-
substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
820
-
substrate: dCMP
Equus asinus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-bromo-dCMP + H2O
-
289003
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289003
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289003
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289003
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289003
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
ara-CMP + H2O
arabinosyl-CMP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
at a very low rate
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
effective substrate in presence of dCTP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
CMP + H2O
poor substrate
289003
Equus asinus
UMP + NH3
-
-
-
?
CMP + H2O
at a low rare, strongly activated by dCTP
289003
Equus asinus
UMP + NH3
-
-
-
?
dCMP + H2O
not a substrate of the T form, dTTP-induced form of the enzyme
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
dCMP + H2O
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
dCMP-Hg-S-CH2-CH2-OH + H2O
better substrate of T form: in the presence of dTTP
289003
Equus asinus
dUMP-Hg-S-CH2-CH2-OH + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
hexamer
6 identical subunits
Equus asinus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Equus asinus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ammonium sulfate
substrate dCMP-Hg-S-CH2-CH2-OH, 0.2 M ammonium sulfate: activation, reversed by cCTP
Equus asinus
dCTP
allosteric activator; allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dTTP
allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH, at relatively high, 1 to 2 mM, dCMP-Hg-S-CH2-CH2-OH concentration
Equus asinus
Glutaraldehyde
substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition
Equus asinus
General Stability (protein specific)
General Stability
Organism
dCTP or glycerol stabilize
Equus asinus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5-Hg-dCMP
irreversible competitive inhibitor, in the absence of mercaptoethanol
Equus asinus
dAMP
competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate
Equus asinus
dCTP
allosteric activator, 12fold, for aminohydrolysis of dCMP, inhibitor, 50% inhibition, for substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dGMP
competitive inhibitor
Equus asinus
dTTP
allosteric inhibitor; allosteric inhibitor, 100% inhibition, for substrate dCMP, becomes activator, 2.5fold, for mercury substrate: 5-Hg-dCMP in the presence of mercaptoethanol, dCMP-Hg-S-CH2-CH2-OH
Equus asinus
dUMP
competitive inhibitor
Equus asinus
Glutaraldehyde
25% glutaraldehyde: rapid inhibition; substrate dCMP: rapid inhibition, protection by dGMP, or dTTP or both is slight, substrate dCMP-Hg-S-CH2-CH2-OH: 3fold activation
Equus asinus
glycerol
substrate dCMP: activation, substrate dCMP-Hg-S-CH2-CH2-OH, 20% glycerol: inhibition, removed by dTTP
Equus asinus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic experiments; kinetic properties and parameters
Equus asinus
0.26
-
dCMP-Hg-S-CH2-CH2-OH
-
Equus asinus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
120000
-
-
Equus asinus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dCMP + H2O
Equus asinus
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
dUMP + NH3
-
Equus asinus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Equus asinus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spleen
-
Equus asinus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Equus asinus
23
-
substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
820
-
substrate: dCMP
Equus asinus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-bromo-dCMP + H2O
-
289003
Equus asinus
5-bromo-dUMP + NH3
-
-
-
?
5-fluoro-dCMP + H2O
-
289003
Equus asinus
5-fluoro-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
deamination activated by dTTP and inhibited by dCTP
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-Hg-dCMP + H2O
5-mercury derivative of dCMP only in the presence of an excess of mercaptoethanol
289003
Equus asinus
5-Hg-dUMP + NH3
-
-
-
?
5-hydroxymethyl-dCMP + H2O
-
289003
Equus asinus
5-hydroxymethyl-dUMP + NH3
-
-
-
?
5-iodo-dCMP + H2O
-
289003
Equus asinus
5-iodo-dUMP + NH3
-
-
-
?
5-methyl-dCMP + H2O
-
289003
Equus asinus
5-methyl-dUMP + NH3
-
-
-
?
ara-CMP + H2O
arabinosyl-CMP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
at a very low rate
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
ara-CMP + H2O
effective substrate in presence of dCTP
289003
Equus asinus
ara-UMP + NH3
-
-
-
?
CMP + H2O
poor substrate
289003
Equus asinus
UMP + NH3
-
-
-
?
CMP + H2O
at a low rare, strongly activated by dCTP
289003
Equus asinus
UMP + NH3
-
-
-
?
dCMP + H2O
not a substrate of the T form, dTTP-induced form of the enzyme
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
dCMP + H2O
catalyzed reaction is at a branching point of the pathway leading to dCTP and dTTP, immediate pyrimidine deoxynucleotide precursors in DNA biosynthesis
289003
Equus asinus
dUMP + NH3
-
289003
Equus asinus
?
dCMP-Hg-S-CH2-CH2-OH + H2O
better substrate of T form: in the presence of dTTP
289003
Equus asinus
dUMP-Hg-S-CH2-CH2-OH + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 identical subunits
Equus asinus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
assay at
Equus asinus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at, substrate: dCMP-Hg-S-CH2-CH2-OH
Equus asinus
Other publictions for EC 3.5.4.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734322
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1
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2
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1
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8
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1
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1
1
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711628
Li
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158
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688405
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J. Mol. Biol.
377
220-231
2008
1
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1
1
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1
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5
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1
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1
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686248
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Involvement of deoxycytidylate ...
Saccharomyces cerevisiae
Curr. Biol.
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688732
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Paramecium bursaria Chlorella virus 1
J. Virol.
81
7662-7671
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1
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1
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3
2
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5
1
2
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4
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1
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4
1
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1
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2
2
1
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5
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1
1
1
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2
2
1
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667587
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Three-dimensional structure of ...
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Biochemistry
43
13715-13723
2004
1
1
1
1
1
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2
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1
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1
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1
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669680
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Homo sapiens
J. Gen. Virol.
84
1437-1441
2003
1
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3
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1
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670328
Liou
Assessment of the effect of ph ...
Homo sapiens
Mol. Pharmacol.
63
105-110
2003
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10
1
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1
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6
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6
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667541
Cha
Modifications of deoxycytidine ...
Rattus norvegicus
Biochem. Pharmacol.
63
717-723
2002
1
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1
4
-
-
-
1
-
1
-
-
-
-
-
3
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
-
3
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
668584
Jost
5-Methyldeoxycytidine monophos ...
Homo sapiens
FEBS Lett.
519
128-134
2002
1
-
-
-
-
-
1
1
-
1
-
3
-
2
-
-
1
-
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
1
-
3
-
-
-
1
-
2
-
-
5
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289015
Keefe
A T4-phage deoxycytidylate dea ...
Enterobacteria phage T2, Enterobacteria phage T6, Escherichia virus T4
J. Biol. Chem.
275
12598-12602
2000
7
-
1
-
3
4
4
5
-
4
9
2
-
4
-
-
1
3
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
7
-
1
-
-
3
4
-
4
-
5
-
4
9
2
-
-
-
1
1
-
4
2
4
5
1
-
-
5
2
-
-
-
-
-
-
-
-
-
289014
Nucci
Interaction of the high-affini ...
Equus asinus
Arch. Biochem. Biophys.
310
49-53
1994
2
1
-
-
-
-
3
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289017
Moore
Identification of a site neces ...
Escherichia virus T4, Homo sapiens
Biochemistry
33
2104-2112
1994
3
-
2
-
1
-
6
-
-
1
-
2
-
2
-
-
1
2
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
3
-
2
-
-
1
-
-
6
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
289018
Maley
Properties of an affinity-colu ...
Homo sapiens
Biochim. Biophys. Acta
1162
161-170
1993
2
-
1
-
-
5
11
-
-
7
2
1
-
3
-
-
1
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
2
-
1
-
-
-
5
-
11
-
-
-
7
2
1
-
-
-
1
-
1
1
1
10
1
1
-
-
1
2
-
-
-
-
-
-
-
-
-
289016
Xu
Modulation of deoxycytidylate ...
Homo sapiens
Biochem. Pharmacol.
44
1819-1827
1992
1
1
-
-
-
-
7
-
-
-
-
2
-
2
-
-
1
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
7
-
-
-
-
-
2
-
-
-
1
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289012
Nucci
Allosteric modifier and substr ...
Equus asinus
Arch. Biochem. Biophys.
289
19-25
1991
2
-
-
-
-
1
4
1
-
-
2
1
-
3
-
-
1
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
4
-
1
-
-
2
1
-
-
-
1
-
3
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
289013
Whitehead
Hill coefficient ratios give b ...
Equus asinus
Arch. Biochem. Biophys.
289
12-18
1991
2
-
-
-
-
-
2
1
-
-
-
1
-
1
-
-
1
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289019
Riva
-
Correlation between deoxycytid ...
Homo sapiens
J. Cell. Pharmacol.
1
79-85
1990
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289020
Maley
A tale of two enzymes, deoxycy ...
Bacillus subtilis, Enterobacteria phage T2, Enterobacteria phage T6, Equus asinus, Escherichia virus T4, Gallus gallus, Herpes simplex virus, Homo sapiens, Macaca mulatta polyomavirus 1, no activity in Escherichia coli, no activity in Salmonella typhimurium, Polyomavirus sp., Rattus norvegicus, Saccharomyces cerevisiae, Sea urchin
Prog. Nucleic Acid Res. Mol. Biol.
39
49-80
1990
10
3
1
1
-
6
8
8
-
-
1
28
-
15
-
-
2
8
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
10
3
1
-
1
-
6
-
8
-
8
-
-
1
28
-
-
-
2
-
20
1
-
70
7
2
-
-
-
-
-
-
-
-
-
-
-
-
-
288997
Maley
Probing the infra-structure of ...
Enterobacteria phage T2, Gallus gallus
Adv. Enzyme Regul.
22
413-430
1984
3
-
-
1
-
-
-
2
-
-
-
8
-
5
-
-
1
2
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
2
-
-
-
8
-
-
-
1
-
1
-
-
10
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289000
McIntosh
Isolation of a Saccharomyces c ...
no activity in Escherichia coli, no activity in Salmonella typhimurium, Saccharomyces cerevisiae
J. Bacteriol.
158
644-649
1984
1
-
-
-
-
-
-
2
-
1
-
1
-
5
-
-
1
-
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
-
-
1
-
-
-
1
9
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
209853
Neale
Enzymes of pyrimidine deoxyrib ...
Mycoplasma mycoides
J. Bacteriol.
156
1001-1005
1983
1
-
-
-
-
1
1
2
-
-
-
1
-
3
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
2
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289002
Mastrantonio
Analysis of competition for su ...
Equus asinus
Eur. J. Biochem.
137
421-427
1983
2
-
-
-
-
-
3
3
-
-
1
1
-
2
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
3
-
-
1
1
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289003
Raia
Reversal of the effect of the ...
Equus asinus
J. Mol. Biol.
157
557-570
1982
5
-
-
-
-
1
8
2
-
-
1
1
-
1
-
-
1
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
5
-
-
-
-
-
1
-
8
-
2
-
-
1
1
-
-
-
1
-
1
3
-
15
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288999
Ellims
Deoxycytidylate deaminase. Pur ...
Homo sapiens
J. Biol. Chem.
256
6335-6340
1981
7
-
-
-
-
3
16
1
-
4
2
2
-
2
-
-
1
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
7
-
-
-
-
-
3
-
16
-
1
-
4
2
2
-
-
-
1
-
2
1
1
8
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
288998
Nucci
Freezing of dCMP aminohydrolas ...
Equus asinus
J. Mol. Biol.
124
133-145
1978
2
-
-
-
-
-
3
1
-
1
2
1
-
1
-
-
1
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
3
-
1
-
1
2
1
-
-
-
1
-
1
2
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
289004
Maley
Deoxycytidylate deaminase from ...
Enterobacteria phage T2, no activity in Escherichia coli
Methods Enzymol.
51
412-418
1978
2
-
-
-
-
3
1
4
-
4
1
2
-
3
-
-
1
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
2
-
-
-
-
-
3
-
1
-
4
-
4
1
2
-
-
-
1
-
-
1
1
12
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
289005
Mollgaard
Deoxycytidylate deaminase from ...
Bacillusphage phiE, Bacillus subtilis, Bacillus subtilis ED40, bacteriophage SP8, no activity in Escherichia coli, no activity in Salmonella typhimurium
J. Biol. Chem.
253
3536-3542
1978
9
-
-
-
-
4
2
2
-
10
1
2
-
7
-
-
1
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
9
-
-
-
-
-
4
-
2
-
2
-
10
1
2
-
-
-
1
-
-
1
3
12
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289006
Dosseva.I.M.; Tomov
-
Stabilizing effect of ethylene ...
Mus musculus
Dokl. Bolg. Akad. Nauk
28
241-244
1975
-
-
-
-
-
5
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
289007
Rolton
Deoxycytidylate deaminase. Evi ...
Cricetinae, Herpes simplex virus
Biochem. J.
143
403-409
1974
6
-
-
-
-
3
6
4
-
2
-
2
-
2
-
-
2
-
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
6
-
-
-
-
-
3
-
6
-
4
-
2
-
2
-
-
-
2
-
2
-
-
4
-
2
-
4
-
2
-
-
-
-
-
-
-
-
-
289008
Rolton
Deoxycytidylate deaminase. Pro ...
Cricetinae
Biochem. J.
141
211-217
1974
1
-
-
-
-
3
2
1
-
3
2
1
-
1
-
-
1
1
-
2
1
2
4
-
1
-
2
-
1
1
-
1
-
-
-
1
-
-
1
-
-
3
-
2
-
1
-
3
2
1
-
-
-
1
-
2
1
2
4
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
289009
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Escherichia virus T4, Gallus gallus
J. Biol. Chem.
247
931-939
1972
5
-
-
-
-
2
3
4
-
-
1
3
-
3
-
-
2
3
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
5
-
-
-
-
-
2
-
3
-
4
-
-
1
3
-
-
-
2
-
1
1
2
11
3
2
-
-
-
4
-
-
-
-
-
-
-
-
-
289010
Maley
T2r+ bacteriophage-induced enz ...
Enterobacteria phage T2, Gallus gallus
J. Biol. Chem.
247
940-945
1972
3
-
-
-
-
2
3
-
-
-
2
2
-
2
-
-
-
2
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
3
-
-
-
-
2
2
-
-
-
-
1
1
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
289011
Sergott
On the regulation of a bacteri ...
Lactobacillus acidophilus, Staphylococcus aureus
J. Biol. Chem.
246
7755-7758
1971
4
-
-
-
-
1
6
-
-
-
-
2
-
2
-
-
2
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
6
-
-
-
-
-
2
-
-
-
2
-
-
1
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-