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Literature summary for 3.5.4.12 extracted from

  • Mastrantonio, S.; Nucci, R.; Vaccaro, C.; Rossi, M.; Whitehead, E.P.
    Analysis of competition for substrate sites in an allosteric enzyme with co-operative kinetics. Effects of dAMP and dUMP on donkey spleen deoxycytidylate aminohydrolase (1983), Eur. J. Biochem., 137, 421-427.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dAMP at low substrate, dCMP, concentration: activation at higher concentration of either: inhibition Equus asinus
dCTP allosteric activator Equus asinus
dCTP allosteric activator, increases substrate affinity Equus asinus

Inhibitors

Inhibitors Comment Organism Structure
dAMP at low substrate, dCMP, concentration: activation at higher concentration of either: competitive inhibition; competitive inhibitor, activates enzyme at low substrate concentration mimicing the cooperative effect of the substrate Equus asinus
dTTP allosteric inhibitor Equus asinus
dUMP competitive inhibition, kinetics and mechanism of inhibition Equus asinus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic studies, steady-state kinetics, allosteric kinetics Equus asinus
0.024
-
dCMP in the presence of dCTP Equus asinus
0.037
-
dCMP in the presence of dCTP Equus asinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
-
Equus asinus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dCMP + H2O Equus asinus
-
dUMP + NH3
-
?

Organism

Organism UniProt Comment Textmining
Equus asinus
-
donkey
-

Reaction

Reaction Comment Organism Reaction ID
dCMP + H2O = dUMP + NH3 mechanism Equus asinus
dCMP + H2O = dUMP + NH3 regulation, allosteric end-product regulation Equus asinus
dCMP + H2O = dUMP + NH3 highly regulated allosteric enzyme Equus asinus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Equus asinus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dCMP + H2O
-
Equus asinus dUMP + NH3
-
?

Subunits

Subunits Comment Organism
hexamer 6 identical subunits Equus asinus