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Literature summary for 3.5.4.10 extracted from
- Experimental characterization of two archaeal inosine 5'-monophosphate cyclohydrolases (2019), PLoS ONE, 14, e0223983 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed in Rosetta2 (DE3) cells | Thermococcus kodakarensis |
| overexpressed in Rosetta2 (DE3) cells | Archaeoglobus fulgidus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00156 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Thermococcus kodakarensis |  |
| 0.0078 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Thermococcus kodakarensis | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Archaeoglobus fulgidus | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Archaeoglobus fulgidus ATCC 49558 | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Thermococcus kodakarensis ATCC BAA-918 | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | O28464 | - |
- |
| Archaeoglobus fulgidus ATCC 49558 | O28464 | - |
- |
| Thermococcus kodakarensis | Q5JD29 | - |
- |
| Thermococcus kodakarensis ATCC BAA-918 | Q5JD29 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus kodakarensis |
- |
Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Thermococcus kodakarensis | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Archaeoglobus fulgidus | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Archaeoglobus fulgidus ATCC 49558 | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus ATCC 49558 | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Thermococcus kodakarensis ATCC BAA-918 | IMP + H2O | - |
? | |
| 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis ATCC BAA-918 | IMP + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AF1811 | locus name | Archaeoglobus fulgidus |
| PurH2 | - |
Archaeoglobus fulgidus |
| PurH2-type IMP cyclohydrolase | - |
Archaeoglobus fulgidus |
| PurO | - |
Thermococcus kodakarensis |
| PurO-type IMP cyclohydrolase | - |
Thermococcus kodakarensis |
| TK0430 | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Archaeoglobus fulgidus |
| 70 | - |
- |
Thermococcus kodakarensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.48 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Thermococcus kodakarensis | |
| 1.32 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis |
| metabolism | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 307.7 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus | |
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