Cloned (Comment) | Organism |
---|---|
overexpressed in Rosetta2 (DE3) cells | Thermococcus kodakarensis |
overexpressed in Rosetta2 (DE3) cells | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00156 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Thermococcus kodakarensis | |
0.0078 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Thermococcus kodakarensis | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Archaeoglobus fulgidus | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Archaeoglobus fulgidus ATCC 49558 | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Thermococcus kodakarensis ATCC BAA-918 | the enzyme catalyze the final step of de novo purine biosynthesis | IMP + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28464 | - |
- |
Archaeoglobus fulgidus ATCC 49558 | O28464 | - |
- |
Thermococcus kodakarensis | Q5JD29 | - |
- |
Thermococcus kodakarensis ATCC BAA-918 | Q5JD29 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Thermococcus kodakarensis | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Archaeoglobus fulgidus | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Archaeoglobus fulgidus ATCC 49558 | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus ATCC 49558 | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Thermococcus kodakarensis ATCC BAA-918 | IMP + H2O | - |
? | |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis ATCC BAA-918 | IMP + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AF1811 | locus name | Archaeoglobus fulgidus |
PurH2 | - |
Archaeoglobus fulgidus |
PurH2-type IMP cyclohydrolase | - |
Archaeoglobus fulgidus |
PurO | - |
Thermococcus kodakarensis |
PurO-type IMP cyclohydrolase | - |
Thermococcus kodakarensis |
TK0430 | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Archaeoglobus fulgidus |
70 | - |
- |
Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Thermococcus kodakarensis | |
1.32 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyze the final step of de novo purine biosynthesis | Thermococcus kodakarensis |
metabolism | the enzyme catalyze the final step of de novo purine biosynthesis | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
307.7 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.3, 50°C | Archaeoglobus fulgidus |