Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
20 mg/ml purified recombinant enzyme in 20 mM Tris, pH 7.9, 150 mM NaCl, 0.25 mM TCEP, nanodroplet vapor diffusion method, the crystallization solution contains 20% w/v PEG 6000 and 0.1 M citrate pH 5.0, 10% v/v PEG 200 as a cryoprotectant, X-ray diffraction structure determination and anaylsis at 1.88 A resolution, modelling | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49700 | - |
1 * 49700, enzyme comprising residues 1-452, i.e. the IMP cyclohydrolase part of the bifunctional enzyme, SDS-PAGE | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermotoga maritima | the TM1249 gene or gene purH of Thermotoga maritima encodes a bifunctional enzyme with 5-aminoimidazole-4-carboxamide ribonucleotide transformylase, AICAR Tfase, EC 2.1.2.3, and inosine 5'-monophosphate cyclohydrolase, IMPCH, EC 3.5.4.10, activities. These activities represent the final two steps of the de novo purine biosynthesis pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X0X6 | gene TM1249 or purH | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formaminoimidazole-4-carboxamide ribonucleotide | the IMPCH domain catalyzes the intramolecular cyclization of 5-formyl-AICAR, FAICAR, to IMP | Thermotoga maritima | IMP + H2O | - |
? | |
additional information | the TM1249 gene or gene purH of Thermotoga maritima encodes a bifunctional enzyme with 5-aminoimidazole-4-carboxamide ribonucleotide transformylase, AICAR Tfase, EC 2.1.2.3, and inosine 5'-monophosphate cyclohydrolase, IMPCH, EC 3.5.4.10, activities. These activities represent the final two steps of the de novo purine biosynthesis pathway, overview | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49700, enzyme comprising residues 1-452, i.e. the IMP cyclohydrolase part of the bifunctional enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
AICAR transformylase/inosine 5'-monophosphate cyclohydrolase | - |
Thermotoga maritima |
IMPCH | - |
Thermotoga maritima |
inosine 5'-monophosphate cyclohydrolase | - |
Thermotoga maritima |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermotoga maritima | sequence calculation | - |
5.49 |