Any feedback?
Literature summary for 3.5.4.10 extracted from
- Catalytic mechanism of the cyclohydrolase activity of human aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase (2004), Biochemistry, 43, 1184-1192.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D125A | 10fold increase in KM-value | Homo sapiens |
| D125E | 5fold increase in Km-value | Homo sapiens |
| D125N | 3fold increase in Km-value | Homo sapiens |
| K137A | 30fold increase in KM-value, 30fold decrease in kcat-value | Homo sapiens |
| K137R | 3fold increase in KM-value15fold decrease in kcat-value | Homo sapiens |
| K66A | more than 100fold increase in KM-value | Homo sapiens |
| Y104A | decrease in kcat-value by one orders of magnitude | Homo sapiens |
| Y104A/D125A | no enzymic activity | Homo sapiens |
| Y104F | decrease in kcat-value by two orders of magnitude | Homo sapiens |
| Y104F/D125A | no enzymice activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, wild-type | Homo sapiens |  |
| 0.0027 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant D125N | Homo sapiens | |
| 0.0027 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant K137R | Homo sapiens | |
| 0.003 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant Y104A | Homo sapiens | |
| 0.0036 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant Y104F | Homo sapiens | |
| 0.0052 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant D125E | Homo sapiens | |
| 0.0091 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant D125A | Homo sapiens | |
| 0.031 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant K137A | Homo sapiens | |
| 0.115 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant K66A | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
bifunctional aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolase | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | - |
Homo sapiens | IMP + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutantD125A | Homo sapiens | |
| 0.036 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutantY104F | Homo sapiens | |
| 0.062 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutantK66A | Homo sapiens | |
| 0.136 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutantY104A | Homo sapiens | |
| 0.147 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant D125E | Homo sapiens | |
| 0.276 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutantK137A | Homo sapiens | |
| 0.507 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant K137R | Homo sapiens | |
| 0.603 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, mutant D125N | Homo sapiens | |
| 8.6 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | pH 7.5, 22°C, wild-type | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
