Literature summary for 3.5.4.1 extracted from

Leonhardt, N.; Divol, F.; Chiarenza, S.; Deschamps, S.; Renaud, J.; Giacalone, C.; Laurent, N.; Berthome, R.; Peret, B.
Tissue-specific inactivation by cytosine deaminase/uracil phosphoribosyl transferase as a tool to study plant biology (2019), Plant J., 101, 731-741 .

Search for more literature for 3.5.4.1

Application

Application	Comment	Organism
agriculture	construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a fusion protein of fluorocytosine deaminase FCY with the bacterial uracil phosphoribosyl transferase (UPP) gene. The recombinant protein converts the precursor 5-fluorocytosine into 5-fluorouracyl, used in the treatment of a range of cancers. The FCY-UPP gene construct acts in a cell-autonomous manner and can inactivate slow developmental processes like lateral root formation by targeting pericycle cells. The 5-fluorouracil precursor acts systemically the tissular inactivation is reversible, and can be used to synchronize plant responses or to determine cell type-specific functions during different developmental stages	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	Q12178	-	-
Saccharomyces cerevisiae ATCC 204508	Q12178	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	-	Saccharomyces cerevisiae	5-fluorouracil + NH3	-	?
5-fluorocytosine + H2O	-	Saccharomyces cerevisiae ATCC 204508	5-fluorouracil + NH3	-	?

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Release 2023.1 (January 2023)