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Literature summary for 3.5.4.1 extracted from

  • Ireton, G.C.; McDermott, G.; Black, M.E.; Stoddard, B.L.
    The structure of Escherichia coli cytosine deaminase (2002), J. Mol. Biol., 315, 687-697.
    View publication on PubMed
Search for more literature for 3.5.4.1

Application

Application Comment Organism
medicine enzyme is of interest both for antimicrobial drug design and for gene therapy applications against tumors Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
10 mg/ml purified recombinant His-tagged enzyme, vapour phase equilibration against 11-14% PEG 8000, 0.1 M HEPES, pH 7.3-7.7, 0.2 M MgCl2, in hanging drop geometry, flash-cooling in a buffer containing 30% v/v glycerol for cryoprotection, also crystallization of a seleno-methionine derivatized enzyme, X-ray diffraction structure determination and analysis at 1.5 A resolution Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to the active site, the metal ion coordinates a hydroxyl nucleophile, binding structure, overview Escherichia coli
Zn2+ enzyme bound, binding structure, overview Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 6 * 48000, (alphabeta)8 barrel structure, crystal structure Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli enzyme is an important member of the pyrimidine salvage pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P25524
-
-

Reaction

Reaction Comment Organism Reaction ID
cytosine + H2O = uracil + NH3 substrate binding involves a significant conformational change that sequesters the reaction complex from solvent, active site architecture and substrate binding, catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O low activity Escherichia coli 5-fluorouracil + NH3
-
 ?
cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
additional information enzyme is an important member of the pyrimidine salvage pathway Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 48000, (alphabeta)8 barrel structure, crystal structure Escherichia coli
More stabilized by a unique domain-swapping interaction between enzyme subunits, the active site is located in the mouth of the enzyme barrel Escherichia coli

Synonyms

Synonyms Comment Organism
CD
-
 Escherichia coli