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Literature summary for 3.5.3.9 extracted from
- Molecular and functional characterization of allantoate amidohydrolase from Phaseolus vulgaris (2014), Physiol. Plant., 152, 43-58.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Nicotiana benthamiana and Escherichia coli strain BL21(DE3) | Phaseolus vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
Allantoate | at pH 8.0 and 40°C | Phaseolus vulgaris |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, mature enzyme, SDS-PAGE | Phaseolus vulgaris |
| 52400 | - |
x * 52400, calculated from amino acid sequence | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allantoate + H2O | Phaseolus vulgaris | - |
(S)-ureidoglycine + NH3 + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | A6YS26 | cultivar Great Northern | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| StrepTactin Sepharose column chromatography | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hairy root | - |
Phaseolus vulgaris | - |
| leaf | - |
Phaseolus vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allantoate + H2O | - |
Phaseolus vulgaris | (S)-ureidoglycine + NH3 + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, mature enzyme, SDS-PAGE | Phaseolus vulgaris |
| ? | x * 52400, calculated from amino acid sequence | Phaseolus vulgaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAH | - |
Phaseolus vulgaris |
| allantoate amidohydrolase | - |
Phaseolus vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Phaseolus vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | - |
Allantoate | at pH 8.0 and 40°C | Phaseolus vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Phaseolus vulgaris |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | main enzyme involved in allantoate degradation in common bean | Phaseolus vulgaris |
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