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Literature summary for 3.5.3.9 extracted from

  • Werner, A.K.; Sparkes, I.A.; Romeis, T.; Witte, C.P.
    Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean (2008), Plant Physiol., 146, 418-430.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.9

Cloned(Commentary)

Cloned (Comment) Organism
transient expression in Nicotiana tabacum as StrepII-tagged enzyme using the Agrobacterium tumefaciens transfection method Glycine max
transient expression in Nicotiana tabacum as StrepII-tagged enzyme using the Agrobacterium tumefaciens transfection method, co-expression of YFP-tagged enzyme in Nicotiana tabacum plants with endoplasmic reticulum and peroxisomal markers Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information an N-terminally truncated version of AAH-YFP lacking the first 71 amino acids exclusively localized to the cytosol Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Borate
-
 Arabidopsis thaliana
Borate
-
 Glycine max
fluoride
-
 Arabidopsis thaliana
fluoride
-
 Glycine max
L-Asn inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition Arabidopsis thaliana
L-Asn inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition Glycine max
L-Asp inhibition via chelating Mn2+, allantoate protects and reverses Arabidopsis thaliana
L-Asp inhibition via chelating Mn2+, allantoate protects and reverses Glycine max
additional information no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate Arabidopsis thaliana
additional information no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
endoplasmic reticulum
-
 Glycine max 5783
-
Golgi apparatus
-
 Arabidopsis thaliana 5794
-
Golgi apparatus
-
 Glycine max 5794
-
peroxisome
-
 Arabidopsis thaliana 5777
-
peroxisome
-
 Glycine max 5777
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Arabidopsis thaliana
Mn2+ required for activity Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51900
-
 x * 51900, SDS-PAGE Glycine max
53100
-
 x * 53100, SDS-PAGE Arabidopsis thaliana
100000
-
 gel filtration Glycine max
103000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
allantoate + 2 H2O Arabidopsis thaliana allantoate degradation is required to recycle purine-ring nitrogen in plants (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
allantoate + 2 H2O Glycine max allantoate degradation is required to recycle purine-ring nitrogen in plants (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O49434 ecotype Columbia
-
Glycine max A9GYV1 cv. ‘Williams 82’, gene aah
-

Purification (Commentary)

Purification (Comment) Organism
recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography Arabidopsis thaliana
recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
leaf
-
 Glycine max
-
additional information distribution, overview Arabidopsis thaliana
-
additional information distribution, overview Glycine max
-
root
-
 Arabidopsis thaliana
-
root
-
 Glycine max
-
seedling
-
 Arabidopsis thaliana
-
seedling
-
 Glycine max
-
shoot
-
 Arabidopsis thaliana
-
shoot
-
 Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allantoate + 2 H2O allantoate degradation is required to recycle purine-ring nitrogen in plants Arabidopsis thaliana (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
allantoate + 2 H2O allantoate degradation is required to recycle purine-ring nitrogen in plants Glycine max (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
allantoate + 2 H2O an ureide Arabidopsis thaliana (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
allantoate + 2 H2O an ureide Glycine max (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
additional information substrate specificity, overview, no activity with N-alpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly Arabidopsis thaliana ?
-
 ?
additional information substrate specificity, overview, no activity with N-lpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly Glycine max ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53100, SDS-PAGE Arabidopsis thaliana
? x * 51900, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
AAH
-
 Arabidopsis thaliana
AAH
-
 Glycine max
allantoate amidohydrolase
-
 Arabidopsis thaliana
allantoate amidohydrolase
-
 Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana
30
-
 assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Arabidopsis thaliana
8
-
 assay at Glycine max

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.032
-
 pH 8.0, 30°C Arabidopsis thaliana fluoride
0.0425
-
 pH 8.0, 30°C Glycine max fluoride