Application | Comment | Organism |
---|---|---|
analysis | development of an enzyme cycling method for measuring allantoin concentrations in human serum | Metabacillus fastidiosus |
General Stability | Organism |
---|---|
preparation of allantoate amidohydrolase stabilized by the addition of 0.1 mM EDTA | Metabacillus fastidiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | essential for enzyme activity, catalyzes the second step of the developed assay, ammonia removal reaction in the presence of | Metabacillus fastidiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
allantoate + H2O | Metabacillus fastidiosus | - |
ureidoglycine + NH3 + CO2 | - |
? | |
allantoate + H2O | Metabacillus fastidiosus ATCC 29604 | - |
ureidoglycine + NH3 + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metabacillus fastidiosus | - |
ATCC29604 | - |
Metabacillus fastidiosus ATCC 29604 | - |
ATCC29604 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme cycling method for measuring allantoin concentrations in human serum, ammonia is liberated from allantoate by the activity of allantoate amidohydrolase, 70 microM allantoin and a concentration of 2 U/ml allantoate amidohydrolase required for maximum absorbance, measurement of allantoin levels in healthy subjects | Metabacillus fastidiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allantoate + H2O | - |
Metabacillus fastidiosus | ureidoglycine + NH3 + CO2 | - |
? | |
allantoate + H2O | - |
Metabacillus fastidiosus ATCC 29604 | ureidoglycine + NH3 + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
allantoate amidohydrolase | - |
Metabacillus fastidiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Metabacillus fastidiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Metabacillus fastidiosus |