Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lysine | - |
Phaseolus vulgaris | |
additional information | phenylhydrazine crucial for enzyme activity | Phaseolus vulgaris | |
tryptophan | - |
Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.15 mM completely inhibits incubation mixture with Mn2+ | Phaseolus vulgaris | |
Cu2+ | 0.15 mM completely inhibits incubation mixture with Mn2+ | Phaseolus vulgaris | |
EDTA | abolishes activation of enzyme by Mn2+; final concentration of 2 mM | Phaseolus vulgaris | |
Fe2+ | 0.15 mM completely inhibits incubation mixture with Mn2+ | Phaseolus vulgaris | |
glycolate | 50% activity loss with 0.06 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.06 mM | Phaseolus vulgaris | |
glyoxylate | 50% activity loss with 0.02 mM glycolate for 10 min at 30°C; strong inhibitor, 50% inhibition at 0.02 mM | Phaseolus vulgaris | |
guanidine | 10% and 80% activity loss with 2 M and 5 M L-guanidine pre-treatment for 10 min at 30°C | Phaseolus vulgaris | |
iminourea | pre-treatment with 2mM or 5mM results in a loss of acitivity of 10% or 80% of activity | Phaseolus vulgaris | |
additional information | pre-treatment with N-acetylimidazole, iodoacetic acid or 5,5'-dithiobis-(2-nitrobenzoic acid) has no effect on enzyme activity | Phaseolus vulgaris | |
N-Acetylimidazole | 5.6% activity loss with 10 mM n-acetylimidazole 10 min at 30°C | Phaseolus vulgaris | |
N-bromosuccinimide | 100% activity loss with 10 mM n-bromosuccinimide for 10 min at 30°C; pre-treatment completely inhibits activity | Phaseolus vulgaris | |
pyridoxal 5'-phosphate | pre-treatment with 10 mM completely inhibits activity | Phaseolus vulgaris | |
pyridoxal-5-phosphate | 100% activity loss with 10 mM pyridoxal-5-phosphate for 10 min at 30°C | Phaseolus vulgaris | |
SDS | 50% activity loss with 50 mM SDS pre-treatment for 10 min at 30°C; pre-treatment with 50 mM results in a loss of acitivity of 50% of activity | Phaseolus vulgaris | |
Urea | 5% and 20% activity loss with 2 M and 5 M urea pre-treatment for 10 min at 30°C | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
Allantoate | - |
Phaseolus vulgaris | |
76 | - |
Allantoate | - |
Phaseolus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | can replace Mn2+, 85% of activity measured in the presence of Mn2+ | Phaseolus vulgaris | |
Mn2+ | no enzyme activity without Mn2+, maximal activity at concentrations between 0.1 and 0.5 mM | Phaseolus vulgaris | |
Mn2+ | essential for enzyme activity, maximal activity between 0.1-0.5 mM | Phaseolus vulgaris | |
additional information | Mg2+, Ca2+, Cu2+, Co2+ and Fe2+ can not replace Mn2+ | Phaseolus vulgaris | |
Ni2+ | can replace Mn2+, 32% of activity measured in the presence of Mn2+ | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13500 | - |
gel filtration | Phaseolus vulgaris |
13500 | - |
gel filtration on Sephacryl S-200 column | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
french bean | - |
Phaseolus vulgaris | - |
cv. baishui | - |
Purification (Comment) | Organism |
---|---|
579fold purified ond DEAE-Sephadex column, ADE2 pool | Phaseolus vulgaris |
gel filtration with Sephacryl S-200 and Sephadex A50 columns, and dialysis against Tris-HCl pH 7.5 at 4°C | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Phaseolus vulgaris | - |
leaf | - |
Phaseolus vulgaris | - |
root | - |
Phaseolus vulgaris | - |
seed | - |
Phaseolus vulgaris | - |
seedling | - |
Phaseolus vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0004 | - |
ungerminated seeds | Phaseolus vulgaris |
0.00138 | - |
in crude extract after first purification | Phaseolus vulgaris |
0.00138 | - |
crude extract from seedlings | Phaseolus vulgaris |
0.0018 | - |
6-d-old seedlings | Phaseolus vulgaris |
0.776 | - |
579fold purified enzyme from seedlings | Phaseolus vulgaris |
0.8 | - |
after 579-fold purification | Phaseolus vulgaris |
1 | - |
vmax of partially purified ADE2 pool | Phaseolus vulgaris |
Storage Stability | Organism |
---|---|
-30°C, 2 months, 5% loss of activity | Phaseolus vulgaris |
-30°C, Tris-HCl buffer, pH 7.5, 2 months | Phaseolus vulgaris |
4°C, 1 week, 10% loss of activity | Phaseolus vulgaris |
4°C, Tris-HCl buffer, pH 7.5, 1 week | Phaseolus vulgaris |
55°C, 15 min, 15% loss of activity | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allantoate + 2 H2O | - |
Phaseolus vulgaris | ureidoglycolate + CO2 + 2 NH3 | - |
? | |
allantoate + H2O | - |
Phaseolus vulgaris | ureidoglycine + NH3 + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADE2 | - |
Phaseolus vulgaris |
ALAH | - |
Phaseolus vulgaris |
Allantoate amidinohydrolase | - |
Phaseolus vulgaris |
allantoate amidohydrolase | - |
Phaseolus vulgaris |
allantoate amidohydrolase | ALAH | Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Phaseolus vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
treatment for 15 min reveals a loss of 15% of acitivity | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Phaseolus vulgaris |
8.5 | - |
in tricine/NaOH buffer | Phaseolus vulgaris |