Crystallization (Comment) | Organism |
---|---|
hanging drop method at room temperature, the crystal structure of the enzyme is determined in the presence of the inhibitors N-formimino-L-aspartate and N-guanidino-L-glutaric acid at resolutions of 1.9 and 1.4 A, respectively | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-formimino-L-aspartate | - |
Pseudomonas aeruginosa | |
N-guanidino-L-glutaric acid | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the active site of Pa5106 contains a single zinc atom that is coordinated by His56, His58, His232, and Asp320. The nucleophilic solvent water molecule coordinates with the zinc atom at a distance of 2.0 A and is hydrogen bonded to Asp320 and His269 | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formimidoyl-L-glutamate + H2O | Pseudomonas aeruginosa | - |
L-glutamate + formamide | - |
? | |
N-formimidoyl-L-glutamate + H2O | Pseudomonas aeruginosa ATCC 15692 | - |
L-glutamate + formamide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HU77 | - |
- |
Pseudomonas aeruginosa ATCC 15692 | Q9HU77 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-formimidoyl-L-glutamate + H2O | - |
Pseudomonas aeruginosa | L-glutamate + formamide | - |
? | |
N-formimidoyl-L-glutamate + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | L-glutamate + formamide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HutF | - |
Pseudomonas aeruginosa |
N-formimino-L-glutamate iminohydrolase | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the histidine degradation pathway | Pseudomonas aeruginosa |