Literature summary for 3.5.3.7 extracted from

Lee, S.J.; Kim, d.o..J.; Kim, H.S.; Lee, B.I.; Yoon, H.J.; Yoon, J.Y.; Kim, K.H.; Jang, J.Y.; Im, H.N.; An, D.R.; Song, J.S.; Kim, H.J.; Suh, S.W.
Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily (2011), J. Struct. Biol., 175, 329-338.

Search for more literature for 3.5.3.7

Cloned(Commentary)

Cloned (Comment)	Organism
-	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures of Pseudomonas aeruginosa guanidinobutyrase (GbuA) and guanidinopropionase (GpuA) are determined. Although GbuA and GpuA share a common structural fold of the typical ureohydrolase superfamily, they exhibit significant variations in two active site loops	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
M161Y	mutant showed no detectable enzymatic activity against guanidium compounds	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
10.7	-	4-Guanidinobutyrate	pH 7.9, 30°C, wild-type	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-Guanidinobutyrate + H2O	enzyme shows no activity towards 3-guanidinopropionate	Pseudomonas aeruginosa	4-Aminobutyrate + urea	-	?

Synonyms

Synonyms	Comment	Organism
GbuA	-	Pseudomonas aeruginosa
guanidinobutyrase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
94.7	-	4-Guanidinobutyrate	pH 7.9, 30°C, wild-type	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	assay at	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)