Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, more than 200% of initial activity | Xenopus laevis | |
EGTA | 1 mM, about 200% of initial activity | Xenopus laevis | |
additional information | no activation by EDTA or EGTA | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xenopus laevis |
expression in Escherichia coli | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, about 50% of inhibition, reversed by EDTA | Xenopus laevis | |
Mg2+ | 1 mM, about 50% of inhibition, reversed by EGTA | Xenopus laevis | |
additional information | not inhibitory: Ca2+, Mg2+, EDTA, EGTA | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
Allantoate | pH 7.0, 25°C | Xenopus laevis | |
8.44 | - |
Allantoate | - |
Xenopus laevis | |
8.44 | - |
Allantoate | from kidney | Xenopus laevis | |
250 | - |
Allantoate | pH 7.0, 25°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43700 | - |
x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE | Xenopus laevis |
44700 | - |
x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE | Xenopus laevis |
46500 | - |
x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE | Mus musculus |
47200 | - |
x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9JHX6 | - |
- |
Xenopus laevis | - |
- |
- |
Xenopus laevis | Q9W6S5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
recombinant enzyme | Xenopus laevis |
recombinant enzyme | Mus musculus |
Renatured (Comment) | Organism |
---|---|
inhibition by divalent cations is reversed by excess of chaletors of these cations | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Xenopus laevis | - |
liver | - |
Xenopus laevis | - |
lung | - |
Xenopus laevis | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer, pH 7.0, stable | Xenopus laevis |
-20°C, 50 mM phosphate buffer, pH 7.0, stable | Mus musculus |
4°C, above, rapid loss of activity | Xenopus laevis |
4°C, above, rapid loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-ureidoglycolate | - |
Xenopus laevis | glyoxylate + urea | - |
? | |
allantoate + H2O | - |
Xenopus laevis | (-)-ureidoglycolate + urea | - |
r | |
allantoate + H2O | - |
Xenopus laevis | (S)-ureidoglycolate + urea | - |
? | |
allantoate + H2O | - |
Mus musculus | (S)-ureidoglycolate + urea | - |
? | |
allantoate + H2O | - |
Xenopus laevis | ureidoglycolate + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE | Xenopus laevis |
? | x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
allantoicase | - |
Xenopus laevis |
XAlc | - |
Xenopus laevis |
XAlc | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Xenopus laevis |
50 | 55 | - |
Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 min, loss of about 50% of activity | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Xenopus laevis |
7 | - |
assay at | Mus musculus |