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Literature summary for 3.5.3.3 extracted from

  • Schumann, J.; Boehm, G.; Schumacher, G.; Rudolph, R.; Jaenicke, R.
    Stabilization of creatinase from Pseudomonas putida by random mutagenesis (1993), Protein Sci., 2, 1612-1620.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.3

Protein Variants

Protein Variants Comment Organism
A109V enhanced thermal stability compared to wild-type enzyme Pseudomonas putida
A109V/V355M enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive Pseudomonas putida
A109V/V355M/V182I enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive Pseudomonas putida
V182I no thermal stabilization Pseudomonas putida
V355M enhanced thermal stability compared to wild-type enzyme Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.5
-
 Creatine mutant A109V Pseudomonas putida
13.3
-
 Creatine wild type enzyme Pseudomonas putida
13.9
-
 Creatine mutant V355M Pseudomonas putida
14.7
-
 Creatine mutant V182I Pseudomonas putida
15.6
-
 Creatine mutant A109V/V355M/V182I Pseudomonas putida
16.1
-
 Creatine mutant A109V/V355M Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, SDS-PAGE Pseudomonas putida
92000
-
 gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 wild-type enzyme and mutant enzymes A109V, V355M, V181I, A109V/V355M, A109V/V355M/V182I
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
creatine + H2O
-
 Pseudomonas putida sarcosine + urea
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Pseudomonas putida