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Literature summary for 3.5.3.22 extracted from
- Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis (1995), J. Bacteriol., 177, 3714-3720.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Streptomyces clavuligerus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression as fusion to maltose-binding protein | Streptomyces clavuligerus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Streptomyces clavuligerus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanidinoproclavaminic acid + H2O | Streptomyces clavuligerus | involved in clavulanic acid biosynthesis | proclavaminic acid + urea | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces clavuligerus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Streptomyces clavuligerus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyguanidinoproclavaminic acid + H2O | - |
Streptomyces clavuligerus | deoxyproclavaminic acid + urea | - |
? | |
| guanidinoproclavaminic acid + H2O | best substrate | Streptomyces clavuligerus | proclavaminic acid + urea | - |
? | |
| guanidinoproclavaminic acid + H2O | involved in clavulanic acid biosynthesis | Streptomyces clavuligerus | proclavaminic acid + urea | - |
? | |
| additional information | no substrate inhibition observed, no substrate: D-arginine, L-arginine, N-acetyl-L-arginine | Streptomyces clavuligerus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Streptomyces clavuligerus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Streptomyces clavuligerus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
assay at | Streptomyces clavuligerus |
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