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Literature summary for 3.5.3.18 extracted from

  • Hong, L.; Fast, W.
    Inhibition of human dimethylarginine dimethylaminohydrolase-1 by S-nitroso-L-homocysteine and hydrogen peroxide. Analysis, quantification, and implications for hyperhomocysteinemia (2007), J. Biol. Chem., 282, 34684-34692.
    View publication on PubMed
Search for more literature for 3.5.3.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C274A no detectable activity Homo sapiens
C275A retains a kcat value about half of wild type protein Homo sapiens
H173A no detectable activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information insensitive to hydrogen peroxide Homo sapiens
S-nitroso-L-homocysteine
-
 Homo sapiens
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33440
-
 electrospray ionization mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity resin chromatography and phenyl-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information L-arginine is no substrate Homo sapiens ?
-
 ?
NG,NG-dimethyl-L-arginine + H2O
-
 Homo sapiens dimethylamine + L-citrulline
-
 ?
NG-monomethyl-L-arginine + H2O
-
 Homo sapiens citrulline + methylamine
-
 ?
S-methyl-L-thiocitrulline + H2O
-
 Homo sapiens methanethiol + L-citrulline
-
 ?

Synonyms

Synonyms Comment Organism
DDAH-1 isozyme Homo sapiens
dimethylarginine dimethylaminohydrolase
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 activity decreases at pH values above 10 Homo sapiens