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Literature summary for 3.5.3.18 extracted from

  • Stone, E.M.; Person, M.D.; Costello, N.J.; Fast, W.
    Characterization of a transient covalent adduct formed during dimethylarginine dimethylaminohydrolase catalysis (2005), Biochemistry, 44, 7069 - 7078.
    View publication on PubMed
Search for more literature for 3.5.3.18

Cloned(Commentary)

Cloned (Comment) Organism
cloned into a pET-28a expression vector, N-terminal His6-tagged protein overexpressed in Escherichia coli BL21 (DE3) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C249S Cys249 as the catalytic nucleophile required for intermediate formation Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.143
-
 S-methyl-L-thiocitrulline
-
 Pseudomonas aeruginosa
0.31
-
 NG,NG-dimethyl-L-arginine
-
 Pseudomonas aeruginosa
0.67
-
 NG-methyl-L-arginine
-
 Pseudomonas aeruginosa
1.11
-
 NG-Amino-L-arginine
-
 Pseudomonas aeruginosa
2.3
-
 NG-Hydroxy-L-arginine
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
 by ESI-MS Pseudomonas aeruginosa
30500
-
 by sequence analysis Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4E3
-
-

Purification (Commentary)

Purification (Comment) Organism
wild type to more than 98% homogeneity, by Ni-NTA affinity chromatography, mutant required additional purification step, ion-exchange chromatography followed by affinity chromatography, mutant purified to more than 95% homogeneity Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
additional information bad substrates are creatine, creatinine, argininosuccinate, guanidinoacetate, L-homoarginine, L-canvanine Pseudomonas aeruginosa ?
-
 ?
NG,NG-dimethyl-L-arginine + H2O
-
 Pseudomonas aeruginosa dimethylamine + L-citrulline
-
 ?
NG-amino-L-arginine + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
NG-hydroxy-L-arginine + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
NG-methyl-L-arginine + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
S-methyl-L-thiocitrulline + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Synonyms

Synonyms Comment Organism
DDAH
-
 Pseudomonas aeruginosa
dimethylarginine dimethylaminohydrolase
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 NG-Amino-L-arginine
-
 Pseudomonas aeruginosa
0.31
-
 NG-methyl-L-arginine
-
 Pseudomonas aeruginosa
0.33
-
 NG-Hydroxy-L-arginine
-
 Pseudomonas aeruginosa
0.56
-
 NG,NG-dimethyl-L-arginine
-
 Pseudomonas aeruginosa
0.84
-
 S-methyl-L-thiocitrulline
-
 Pseudomonas aeruginosa