Literature summary for 3.5.3.17 extracted from

Lee, S.J.; Kim, d.o..J.; Kim, H.S.; Lee, B.I.; Yoon, H.J.; Yoon, J.Y.; Kim, K.H.; Jang, J.Y.; Im, H.N.; An, D.R.; Song, J.S.; Kim, H.J.; Suh, S.W.
Crystal structures of Pseudomonas aeruginosa guanidinobutyrase and guanidinopropionase, members of the ureohydrolase superfamily (2011), J. Struct. Biol., 175, 329-338.

Search for more literature for 3.5.3.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structures are determined of Mn2+-bound GpuA (methylated), and 1,6-diaminohexane-bound GpuA (unmethylated)	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
Y157M	Km for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly increased compared to wild-type, kcat for substrates 3-guanidinopropionate or 4-guanidinobutyrate significantly decreased	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
12.8	-	3-guanidinopropionate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa
37.3	-	4-Guanidinobutyrate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa
108	-	3-guanidinopropionate	pH 7.9, 30°C, mutant Y157M unmethylated GpuA	Pseudomonas aeruginosa
285	-	4-Guanidinobutyrate	pH 7.9, 30°C, mutant Y157M unmethylated GpuA	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I6K2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-guanidinopropionate + H2O	-	Pseudomonas aeruginosa	beta-alanine + urea	-	?
4-Guanidinobutyrate + H2O	-	Pseudomonas aeruginosa	4-Aminobutyrate + urea	-	?

Synonyms

Synonyms	Comment	Organism
GpuA	-	Pseudomonas aeruginosa
guanidinopropionase	-	Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	8	4-Guanidinobutyrate	pH 7.9, 30°C, mutant Y157M unmethylated GpuA	Pseudomonas aeruginosa
48.8	-	3-guanidinopropionate	pH 7.9, 30°C, mutant Y157M unmethylated GpuA	Pseudomonas aeruginosa
57.6	-	4-Guanidinobutyrate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa
106	-	3-guanidinopropionate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	-	assay at	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.54	-	4-Guanidinobutyrate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa
8.28	-	3-guanidinopropionate	pH 7.9, 30°C, wild-type unmethylated GpuA	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)