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Literature summary for 3.5.3.15 extracted from

  • Lee, C.; Lin, C.; Liu, Y.; Liu, G.; Liu, J.; Hung, H.
    Molecular interplay between the dimer interface and the substrate-binding site of human peptidylarginine deiminase 4 (2017), Sci. Rep., 7, 42662 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C434A small decrase in kcat/Km value Homo sapiens
D465A residue involved in dimerization, about 30% of wild-type catalytic effciency Homo sapiens
F285AV284A residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
F541A residue at dimer interface, about 50% decrease in kcat/Km value Homo sapiens
F576A residue at dimer interface, about 50% decrease in kcat/Km value Homo sapiens
L279A residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
L279D residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
L279I residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
L6A residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
L6D residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
L6I residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
R372A almost complete loss of activity Homo sapiens
R372K complete loss of activity Homo sapiens
R372Q almost complete loss of activity Homo sapiens
R441A residue involved in dimerization, about 6% of wild-type catalytic effciency Homo sapiens
R639A about 30% decrease in kcat/Km value Homo sapiens
V283A residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V283D residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V283I residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V283T residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V284A residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V284D residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V284I residue at dimer interface. Mutation decreases dimer formation and, consequently, enzyme activity Homo sapiens
V469A residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity Homo sapiens
V469L residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity Homo sapiens
V469T residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity Homo sapiens
W347A residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity Homo sapiens
W347F residue is critical for substrate binding at the active site. Mutation leads to a severe reduction in the catalytic activity Homo sapiens
W548A residue at dimer interface, complete loss of activity Homo sapiens
W548F residue at dimer interface, about 50% decrease in kcat/Km value Homo sapiens
W548K residue at dimer interface, about 50% decrease in kcat/Km value Homo sapiens
Y435A residue involved in dimerization, about 4% of wild-type catalytic effciency Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469T, pH 7.5, 25°C Homo sapiens
0.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548K, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant C434A, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant D465A, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F576A, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279A, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279I, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374Q, pH 7.5, 25°C Homo sapiens
0.5
-
 N-alpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.5, 25°C Homo sapiens
0.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6D, pH 7.5, 25°C Homo sapiens
0.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6I, pH 7.5, 25°C Homo sapiens
0.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283I, pH 7.5, 25°C Homo sapiens
0.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284I, pH 7.5, 25°C Homo sapiens
0.6
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6A, pH 7.5, 25°C Homo sapiens
0.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F541A, pH 7.5, 25°C Homo sapiens
0.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374A, pH 7.5, 25°C Homo sapiens
0.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R639A, pH 7.5, 25°C Homo sapiens
0.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283A, pH 7.5, 25°C Homo sapiens
0.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F285A, pH 7.5, 25°C Homo sapiens
0.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283T, pH 7.5, 25°C Homo sapiens
0.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284D, pH 7.5, 25°C Homo sapiens
1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469L, pH 7.5, 25°C Homo sapiens
1.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548F, pH 7.5, 25°C Homo sapiens
1.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284A, pH 7.5, 25°C Homo sapiens
1.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R441A, pH 7.5, 25°C Homo sapiens
1.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279D, pH 7.5, 25°C Homo sapiens
2.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372Q, pH 7.5, 25°C Homo sapiens
3.2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.5, 25°C Homo sapiens
3.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372A, pH 7.5, 25°C Homo sapiens
5.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469A, pH 7.5, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UM07 isoform Pad4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
 Homo sapiens N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?

Subunits

Subunits Comment Organism
More the dimer interface and the substrate-binding site of PAD4, which consist of the I-loop and the S-loop, respectively, are responsible for substrate binding and dimer stabilization Homo sapiens

Synonyms

Synonyms Comment Organism
PAD4
-
 Homo sapiens
PADI4
-
 Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
43
-
 melting temperature, mutants L6D, L6I Homo sapiens
46
-
 melting temperature, mutant D465A Homo sapiens
51
-
 melting temperature, wild-type Homo sapiens
55
-
 melting temperature, mutant V283T Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469T, pH 7.5, 25°C Homo sapiens
0.07
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372A, pH 7.5, 25°C Homo sapiens
0.1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469L, pH 7.5, 25°C Homo sapiens
0.12
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372Q, pH 7.5, 25°C Homo sapiens
0.6
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548K, pH 7.5, 25°C Homo sapiens
0.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469A, pH 7.5, 25°C Homo sapiens
2.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R441A, pH 7.5, 25°C Homo sapiens
2.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.5, 25°C Homo sapiens
2.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284D, pH 7.5, 25°C Homo sapiens
3.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant D465A, pH 7.5, 25°C Homo sapiens
3.6
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279D, pH 7.5, 25°C Homo sapiens
3.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374Q, pH 7.5, 25°C Homo sapiens
4.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6D, pH 7.5, 25°C Homo sapiens
5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R639A, pH 7.5, 25°C Homo sapiens
5.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279A, pH 7.5, 25°C Homo sapiens
5.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283T, pH 7.5, 25°C Homo sapiens
5.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284A, pH 7.5, 25°C Homo sapiens
6.2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548F, pH 7.5, 25°C Homo sapiens
6.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F285A, pH 7.5, 25°C Homo sapiens
7.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6A, pH 7.5, 25°C Homo sapiens
7.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F541A, pH 7.5, 25°C Homo sapiens
7.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374A, pH 7.5, 25°C Homo sapiens
8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant C434A, pH 7.5, 25°C Homo sapiens
8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283A, pH 7.5, 25°C Homo sapiens
9.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283I, pH 7.5, 25°C Homo sapiens
9.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F576A, pH 7.5, 25°C Homo sapiens
10.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6I, pH 7.5, 25°C Homo sapiens
10.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284I, pH 7.5, 25°C Homo sapiens
11.2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279I, pH 7.5, 25°C Homo sapiens
11.7
-
 N-alpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.5, 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372A, pH 7.5, 25°C Homo sapiens
0.05
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R372Q, pH 7.5, 25°C Homo sapiens
0.1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469L, pH 7.5, 25°C Homo sapiens
0.15
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469A, pH 7.5, 25°C Homo sapiens
0.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V469T, pH 7.5, 25°C Homo sapiens
0.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.5, 25°C Homo sapiens
1.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R441A, pH 7.5, 25°C Homo sapiens
1.9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279D, pH 7.5, 25°C Homo sapiens
2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548K, pH 7.5, 25°C Homo sapiens
3.2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284D, pH 7.5, 25°C Homo sapiens
3.7
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284A, pH 7.5, 25°C Homo sapiens
4.6
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant W548F, pH 7.5, 25°C Homo sapiens
6.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283T, pH 7.5, 25°C Homo sapiens
7.1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R639A, pH 7.5, 25°C Homo sapiens
8.6
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F285A, pH 7.5, 25°C Homo sapiens
8.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant D465A, pH 7.5, 25°C Homo sapiens
9
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6D, pH 7.5, 25°C Homo sapiens
9.3
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374Q, pH 7.5, 25°C Homo sapiens
11
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F541A, pH 7.5, 25°C Homo sapiens
11.1
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant R374A, pH 7.5, 25°C Homo sapiens
11.4
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283A, pH 7.5, 25°C Homo sapiens
12.2
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6A, pH 7.5, 25°C Homo sapiens
13.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279A, pH 7.5, 25°C Homo sapiens
19
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V283I, pH 7.5, 25°C Homo sapiens
20
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant C434A, pH 7.5, 25°C Homo sapiens
21.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L6I, pH 7.5, 25°C Homo sapiens
21.8
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant V284I, pH 7.5, 25°C Homo sapiens
23.4
-
 N-alpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.5, 25°C Homo sapiens
24.5
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant F576A, pH 7.5, 25°C Homo sapiens
28
-
 N-alpha-benzoyl-L-arginine ethyl ester mutant L279I, pH 7.5, 25°C Homo sapiens