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Literature summary for 3.5.3.15 extracted from

  • Liu, Y.L.; Chiang, Y.H.; Liu, G.Y.; Hung, H.C.
    Functional role of dimerization of human peptidylarginine deiminase 4 (PAD4) (2011), PLoS ONE, 6, e21314.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.15

Protein Variants

Protein Variants Comment Organism
D273A/R544A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.35 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.5/sec, quaternary structure: dimer, Kd: 0.68 microM Homo sapiens
D547A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 11.6/sec, quaternary structure: monomer/dimer, Kd: 6.4 microM Homo sapiens
D547E Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.72 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 8.9/sec, quaternary structure: monomer/dimer, Kd: 11.2 microM Homo sapiens
D547N Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.86 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 4.9 microM Homo sapiens
E281A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.45 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.1/sec, quaternary structure: dimer, Kd: 0.1 microM Homo sapiens
R8A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 9.3 microM Homo sapiens
R8A/D547A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.4 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.7/sec, quaternary structure: monomer/dimer, Kd: 3.9 microM Homo sapiens
R8E Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.06 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 7.3/sec, quaternary structure: monomer, Kd: 45.6 microM Homo sapiens
R8E/D547E Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.77 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 3.3/sec, quaternary structure: monomer, Kd: 24 microM Homo sapiens
R8H Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.47 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.3/sec, quaternary structure: dimer, Kd: 0.47 microM Homo sapiens
R8K Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.5 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 10.8/sec, quaternary structure: monomer/dimer, Kd: 10.2 microM Homo sapiens
R8L Km (Nalpha-benzoyl-L-arginine ethyl ester): 1.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.3/sec, quaternary structure: monomer, Kd: 16.8 microM Homo sapiens
R8Q Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.6 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 12.2/sec, quaternary structure: monomer/dimer, Kd: 15.7 microM Homo sapiens
Y237A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.36 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.9/sec, quaternary structure: dimer, Kd: 0.29 microM Homo sapiens
Y237A/E281A Km (Nalpha-benzoyl-L-arginine ethyl ester): 0.38 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 13.2/sec, quaternary structure: dimer, Kd: 0.1 microM Homo sapiens
Y435A Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.33 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 5.9/sec, quaternary structure: monomer, Kd: 30.3 microM Homo sapiens
Y435N Km (Nalpha-benzoyl-L-arginine ethyl ester): 2.73 mM, kcat (Nalpha-benzoyl-L-arginine ethyl ester): 4/sec, quaternary structure: monomer, Kd: 33.8 microM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 Nalpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.4, 25°C Homo sapiens
0.35
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D273A/R544A, pH 7.4, 25°C Homo sapiens
0.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547A, pH 7.4, 25°C Homo sapiens
0.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A, pH 7.4, 25°C Homo sapiens
0.38
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A/E281A, pH 7.4, 25°C Homo sapiens
0.4
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A/D547A, pH 7.4, 25°C Homo sapiens
0.45
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant E281A, pH 7.4, 25°C Homo sapiens
0.47
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A, pH 7.4, 25°C Homo sapiens
0.47
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8H, pH 7.4, 25°C Homo sapiens
0.5
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8K, pH 7.4, 25°C Homo sapiens
0.6
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8Q , pH 7.4, 25°C Homo sapiens
0.72
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547E, pH 7.4, 25°C Homo sapiens
0.86
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547N, pH 7.4, 25°C Homo sapiens
1.06
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E, pH 7.4, 25°C Homo sapiens
1.36
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8L, pH 7.4, 25°C Homo sapiens
2.33
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.4, 25°C Homo sapiens
2.73
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435N, pH 7.4, 25°C Homo sapiens
2.77
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E/D547E, pH 7.4, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Nalpha-benzoyl-L-arginine ethyl ester
-
 Homo sapiens Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?

Subunits

Subunits Comment Organism
dimer dimerization of PAD4 is essential for full enzyme activity and calcium-binding cooperativity Homo sapiens
monomer monomeric PAD4 maintains 25-50% enzyme activity compared to the dimeric enzyme Homo sapiens

Synonyms

Synonyms Comment Organism
PAD4
-
 Homo sapiens
peptidylarginine deiminase 4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E/D547E, pH 7.4, 25°C Homo sapiens
4
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435N, pH 7.4, 25°C Homo sapiens
5.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8L, pH 7.4, 25°C Homo sapiens
5.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y435A, pH 7.4, 25°C Homo sapiens
7.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8E, pH 7.4, 25°C Homo sapiens
8.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547E, pH 7.4, 25°C Homo sapiens
10.08
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8K, pH 7.4, 25°C Homo sapiens
10.7
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A/D547A, pH 7.4, 25°C Homo sapiens
11.6
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547A, pH 7.4, 25°C Homo sapiens
12.1
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant E281A, pH 7.4, 25°C Homo sapiens
12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D547N, pH 7.4, 25°C Homo sapiens
12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8A, pH 7.4, 25°C Homo sapiens
12.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8Q , pH 7.4, 25°C Homo sapiens
13.2
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A/E281A, pH 7.4, 25°C Homo sapiens
13.3
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant R8H, pH 7.4, 25°C Homo sapiens
13.4
-
 Nalpha-benzoyl-L-arginine ethyl ester wild-type, pH 7.4, 25°C Homo sapiens
13.5
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant D273A/R544A, pH 7.4, 25°C Homo sapiens
13.9
-
 Nalpha-benzoyl-L-arginine ethyl ester mutant Y237A, pH 7.4, 25°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens