Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells as a fusion protein with glutathione S-transferase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
WSRYH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.187 | - |
YWRDH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.27 | - |
MWRHV | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.325 | - |
KDRNW | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.345 | - |
WTRGE | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.372 | - |
TKQTARKSTGGK | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.45 | - |
LDRGE | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
0.575 | - |
TSTGGRQGSHH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose 4B column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
filaggrin L-Arg + H2O | - |
Homo sapiens | filaggrin L-citrulline + NH3 | - |
? | |
histone H3 L-arginine + H2O | - |
Homo sapiens | histone H3 L-citrulline + NH3 | - |
? | |
KDRNW + H2O | - |
Homo sapiens | ? | - |
? | |
LDRGE + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination | Homo sapiens | ? | - |
? | |
additional information | no activity toward PVRAK, DQRTA, DVRIA, and EPRLF | Homo sapiens | ? | - |
? | |
MWRHV + H2O | - |
Homo sapiens | ? | - |
? | |
N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Homo sapiens | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
TKQTARKSTGGK + H2O | - |
Homo sapiens | ? | - |
? | |
TSTGGRQGSHH + H2O | - |
Homo sapiens | ? | - |
? | |
WSRYH + H2O | - |
Homo sapiens | ? | - |
? | |
WTRGE + H2O | - |
Homo sapiens | ? | - |
? | |
YWRDH + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAD-4 | - |
Homo sapiens |
peptidylarginine deiminase-4 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
TKQTARKSTGGK | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
1.3 | - |
TSTGGRQGSHH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
1.8 | - |
WSRYH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
2 | - |
MWRHV | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
2 | - |
KDRNW | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
2.3 | - |
YWRDH | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
2.9 | - |
LDRGE | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens | |
3.3 | - |
WTRGE | in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37°C | Homo sapiens |