Cloned (Comment) | Organism |
---|---|
PAD2 and PAD4 from spleen, expression of N-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Homo sapiens | |
EDTA | - |
Mus musculus | |
EDTA | - |
Oryctolagus cuniculus | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide | - |
Homo sapiens | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide | - |
Mus musculus | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide | - |
Oryctolagus cuniculus | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester | complete inhibition of recombinant PAD4 at 2 mM | Homo sapiens | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester | - |
Mus musculus | |
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester | - |
Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Mus musculus | |
Ca2+ | required | Oryctolagus cuniculus | |
Ca2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein-L-arginine + H2O | Mus musculus | - |
protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | Oryctolagus cuniculus | - |
protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | Homo sapiens | - |
protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | Mus musculus C57BL/6 / 129S | - |
protein-L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9Y2J8 | PAD2; isozyme PAD2 | - |
Mus musculus | - |
C57BL/6-129S mice | - |
Mus musculus C57BL/6 / 129S | - |
C57BL/6-129S mice | - |
Oryctolagus cuniculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low activity | Mus musculus | - |
kidney | low activity | Mus musculus | - |
lung | low activity | Mus musculus | - |
additional information | tissue distribution, no activity in testis and liver, overview | Mus musculus | - |
muscle | high activity | Mus musculus | - |
ovary | - |
Mus musculus | - |
skeletal muscle | - |
Oryctolagus cuniculus | - |
spinal cord | - |
Mus musculus | - |
spleen | low activity | Mus musculus | - |
spleen | PAD2 and PAD4 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, calibration using PAD from rabbit skeletal muscle, overview | Oryctolagus cuniculus |
additional information | - |
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Mus musculus |
additional information | - |
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Homo sapiens |
10.14 | - |
muscle enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Mus musculus | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Oryctolagus cuniculus | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Homo sapiens | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
N-alpha-benzoyl-L-arginine ethyl ester + H2O | - |
Mus musculus C57BL/6 / 129S | N-alpha-benzoyl-L-citrulline ethyl ester + NH3 | - |
? | |
protein-L-arginine + H2O | - |
Mus musculus | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | - |
Oryctolagus cuniculus | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | - |
Homo sapiens | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Mus musculus | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Oryctolagus cuniculus | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Homo sapiens | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | - |
Mus musculus C57BL/6 / 129S | protein-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview | Mus musculus C57BL/6 / 129S | protein-L-citrulline + NH3 | - |
? | |
SHQESTRGKSKGKAAAAA + H2O | - |
Homo sapiens | SHQEST-Cit-GKSKGKAAAAA + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAD | - |
Mus musculus |
PAD | - |
Oryctolagus cuniculus |
PAD | - |
Homo sapiens |
peptidylarginine deiminase | - |
Mus musculus |
peptidylarginine deiminase | - |
Oryctolagus cuniculus |
peptidylarginine deiminase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Oryctolagus cuniculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Mus musculus |
7.6 | - |
assay at | Oryctolagus cuniculus |
7.6 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.5 | - |
recombinant PAD4, pH 7.6, 37°C | Homo sapiens | N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester |