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Literature summary for 3.5.3.15 extracted from

  • Lamensa, K.W.E.; Moscarello, M.A.
    Deimination of human myelin basic protein by a peptidylarginine deiminase from bovine brain (1993), J. Neurochem., 61, 987-996.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
benzoyl-Arg ethyl ester Km-value of the high activity binding site Bos taurus
11.2
-
benzoylarginine ethyl ester Km-value of the low activity binding site Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Bos taurus
Ca2+ maximal activity at 2 mM Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein L-Arg + H2O Bos taurus C-1, the most unmodified of the components of myelin basic protein, is an excellent substrate for the enzyme, thereby confirming that the origin of the citrulline in myelin basic protein arises by the action of this enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Arg ethyl ester + H2O
-
Bos taurus benzoyl-citrulline ethyl ester + NH3
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
-
Bos taurus Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
Polyarginine + H2O
-
Bos taurus ?
-
?
protein L-Arg + H2O human myelin basic protein Bos taurus protein L-citrulline + NH3
-
?
protein L-Arg + H2O no activity with histones Bos taurus protein L-citrulline + NH3
-
?
protein L-Arg + H2O C-1, the most unmodified of the components of myelin basic protein, is an excellent substrate for the enzyme, thereby confirming that the origin of the citrulline in myelin basic protein arises by the action of this enzyme Bos taurus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bos taurus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
Bos taurus