Application | Comment | Organism |
---|---|---|
medicine | test for N-amidino-L-aspartate in the urine of uremic patients | Pseudomonas chlororaphis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aspartic acid | competitive inhibition | Pseudomonas chlororaphis | |
Urea | competitive inhibition | Pseudomonas chlororaphis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
N-amidino-L-aspartate | HEPES buffer, pH 8, assay method B | Pseudomonas chlororaphis | |
8.3 | - |
N-amidino-L-aspartate | HEPES buffer, pH 8, assay method A | Pseudomonas chlororaphis | |
20 | - |
N-amidino-L-aspartate | phosphate buffer | Pseudomonas chlororaphis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Pseudomonas putida | |
Co2+ | temperature-dependent activation | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
gel filtration | Pseudomonas chlororaphis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-amidino-L-aspartic acid | Pseudomonas putida | - |
L-aspartic acid + urea | - |
? | |
N-amidino-L-aspartic acid | Pseudomonas chlororaphis | - |
L-aspartic acid + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
fluorescent Pseudomonas | - |
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.73 | - |
- |
Pseudomonas chlororaphis |
Storage Stability | Organism |
---|---|
-15°C, stable for several months | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanidinoglutaric acid + H2O | - |
Pseudomonas chlororaphis | ? | - |
? | |
additional information | - |
Pseudomonas putida | ? | - |
? | |
additional information | enzyme is specific for the L-isomer | Pseudomonas chlororaphis | ? | - |
? | |
additional information | N-amidino-D-aspartate, 3-guanidinopropionic acid, 4-guanidinobutyric acid, 2-amino-4-guanidinopropionic acid, agmatine, arginine, N-acetyl-arginine, creatinine, guanidinoacetic acid are no substrates | Pseudomonas chlororaphis | ? | - |
? | |
N-amidino-L-aspartic acid | - |
Pseudomonas putida | L-aspartic acid + urea | - |
? | |
N-amidino-L-aspartic acid | - |
Pseudomonas chlororaphis | L-aspartic acid + urea | - |
? | |
N-amidino-L-aspartic acid + H2O | - |
Pseudomonas putida | L-aspartic acid + urea | - |
? | |
N-amidino-L-aspartic acid + H2O | - |
Pseudomonas chlororaphis | L-aspartic acid + urea | - |
? | |
N-amidino-L-glutamate + H2O | - |
Pseudomonas putida | L-glutamic acid + urea | - |
? | |
N-amidino-L-glutamate + H2O | - |
Pseudomonas chlororaphis | L-glutamic acid + urea | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min | Pseudomonas chlororaphis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas chlororaphis |