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Literature summary for 3.5.3.12 extracted from

  • Williams, B.J.; Du, R.H.; Calcutt, M.W.; Abdolrasulnia, R.; Christman, B.W.; Blackwell, T.S.
    Discovery of an operon that participates in agmatine metabolism and regulates biofilm formation in Pseudomonas aeruginosa (2010), Mol. Microbiol., 76, 104-119.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa PA14

Protein Variants

Protein Variants Comment Organism
C364G complete loss of activity Pseudomonas aeruginosa PA14
R3G/R4S mutation of the twin-arginine translocation tat-sequence at the N-terminus. Mutant is hardly secreted to periplasm Pseudomonas aeruginosa PA14

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm isoform Agu2A' contains a twin-arginine translocation signal at its N-terminus and is secreted to the periplasm Pseudomonas aeruginosa PA14
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39900
-
 x * 39900, calculated for mature protein Pseudomonas aeruginosa PA14

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa PA14 A0A0H2Z743 isoform agu2A'
-
Pseudomonas aeruginosa PA14 A0A0H2Z8C6 isoform agu2A
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage of translocation signal Pseudomonas aeruginosa PA14

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01055
-
 pH 9.0, 25°C Pseudomonas aeruginosa PA14
0.015
-
 pH 9.0, 37°C Pseudomonas aeruginosa PA14

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O
-
 Pseudomonas aeruginosa PA14 N-carbamoylputrescine + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 39900, calculated for mature protein Pseudomonas aeruginosa PA14

Synonyms

Synonyms Comment Organism
agu2A
-
 Pseudomonas aeruginosa PA14

Expression

Organism Comment Expression
Pseudomonas aeruginosa PA14 presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA' that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase additional information
Pseudomonas aeruginosa PA14 presence of an alternate agmatine operon in the Pseudomonas aeruginosa strain PA14 named agu2ABCA that contains two genes for agmatine deiminases, agu2A and agu2A'. This operon is present in 25% of clinical Pseudomonas aeruginosa isolates. Agmatine induces expression of the operon during the stationary phase of growth and during biofilm growth and agu2ABCA' provides only weak complementation of aguBA, which is induced during log phase up

General Information

General Information Comment Organism
physiological function deletion of the agmatine operon agu2ABCA' reduces biofilm production of strain PA14 following addition of exogenous agmatine Pseudomonas aeruginosa PA14
physiological function deletion of the agmatine operon agu2ABCA', specifically its secreted product Agu2A', reduces biofilm production of strain PA14 following addition of exogenous agmatine Pseudomonas aeruginosa PA14

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.136
-
 agmatine pH 9.0, 25°C Pseudomonas aeruginosa PA14
2.624
-
 agmatine pH 9.0, 37°C Pseudomonas aeruginosa PA14