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Literature summary for 3.5.3.12 extracted from
- Agmatine deiminase from cucumber seedlings is a mono-specific enzyme: purification and characteristics (2003), Protein Expr. Purif., 30, 88-93.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arcaine | agmatine analogue, strong competitive inhibition | Cucumis sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
agmatine | pH 7.0, 30°C | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
| 47000 | - |
x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
| 67000 | - |
gel filtration | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
cv. Tokiwajibae | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | N-glycosylation | Cucumis sativus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2470fold | Cucumis sativus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| agmatine + H2O = N-carbamoylputrescine + NH3 | the enzyme does not show activity of EC 2.1.3.3, ornithine carbamoyltransferase, EC 2.1.3.6, putrescine carbamoyltransferase, and EC 2.7.2.2, carbamate kinase, while putrescine synthase is a multifunctional enzyme, overview | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.5 | - |
purified enzyme | Cucumis sativus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified enzyme, 2 months, stable | Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agmatine + H2O | - |
Cucumis sativus | N-carbamoylputrescine + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | x * 36000, deglycosylated enzyme, SDS-PAGE, x * 47000, glycosylated enzyme, SDS-PAGE, monomer or dimer | Cucumis sativus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| agmatine amidinohydrolase | - |
Cucumis sativus |
| agmatine deiminase | - |
Cucumis sativus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cucumis sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Cucumis sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8 | 89% of maximal activity at pH 6.5, 91% of maximal activity at pH 8.0 | Cucumis sativus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0071 | - |
arcaine | pH 7.0, 30°C | Cucumis sativus | |
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