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Literature summary for 3.5.3.12 extracted from

  • Janowitz, T.; Kneifel, H.; Piotrowski, M.
    Identification and characterization of plant agmatine iminohydrolase, the last missing link in polyamine biosynthesis of plants (2003), FEBS Lett., 544, 258-261.
    View publication on PubMed
Search for more literature for 3.5.3.12

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression as C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C180A site-directed mutagenesis, conserved catalytically relevant Cys, 35% of wild-type activity, inhibition with iodoacetamide is easier abolished by substrate agmatine Arabidopsis thaliana
C366A site-directed mutagenesis, conserved catalytically relevant Cys, 15% of wild-type activity, inhibition with iodoacetamide is easier abolished by substrate agmatine Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide about 70% inhibition at 2 mM, wild-type enzyme Arabidopsis thaliana
NEM 1 mM Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 2 * 43000, recombinant His-tagged wild-type enzyme, SDS-PAGE Arabidopsis thaliana
81000
-
 recombinant His-tagged wild-type enzyme, gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GWW7 gene At5g08170
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
agmatine + H2O = N-carbamoylputrescine + NH3 the plant enzyme also catalyzes the reactions of EC 2.1.3.3, ornithine carbamoyltransferase, EC 2.1.3.6, putrescine carbamoyltransferase, and EC 2.7.2.2, carbamate kinase, thus functioning as a putrescine synthase, converting agmatine and ornithine into putrescine and citrulline, respectively, Cys180 and Cys366 are involved in catalysis but are not essential, residues are located in the substrate binding pocket Arabidopsis thaliana
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26.4
-
 purified recombinant wild-type enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O highly specific for Arabidopsis thaliana N-carbamoylputrescine + NH3
-
 ?
additional information substrate specificity, no activity with guanidinoacetic acid, beta-guanidinopropionic acid, gamma-guanidinobutyric acid, L-arginine, L-citrulline, L-glutamine, L-ornithine, N-carbamoyl-beta-alanine, N-carbamoyl-D,L-aspartic acid, putrescine, spermidine, allylcyanide, beta-cyano-L-alanine, indole-3-acetonitrile, 3-phenylpropionitrile Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, recombinant His-tagged wild-type enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
agmatine deiminase
-
 Arabidopsis thaliana
AIH
-
 Arabidopsis thaliana
More enzyme belongs to the family of C-N-hydrolases Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arabidopsis thaliana