Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
L-arginine | at pH 8.4 and 45°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Bacillus subtilis | - |
L-ornithine + urea | - |
? | |
L-arginine + H2O | Bacillus subtilis 168 | - |
L-ornithine + urea | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39138 | - |
- |
Bacillus subtilis 168 | P39138 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-resin column chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.026 | - |
crude enzyme, at pH 8.4 and 45°C | Bacillus subtilis |
0.15 | - |
after 5.8fold purification, at pH 8.4 and 45°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Bacillus subtilis | L-ornithine + urea | - |
? | |
L-arginine + H2O | - |
Bacillus subtilis 168 | L-ornithine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
L-arginine amidinohydrolase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | more than 50% activity between 30 and 65°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | more than 50% activity between pH 6.0 and 10.0 | Bacillus subtilis |