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Literature summary for 3.5.3.1 extracted from

  • Srivastava, A.; Sau, A.K.
    Biochemical studies on Helicobacter pylori arginase: insight into the difference in activity compared to other arginases (2010), IUBMB Life, 62, 906-915.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.7
-
L-arginine pH 5.0, 37°C, Mn2+-activited enzyme, K0.5 value, Hill-coefficient 1.6 Helicobacter pylori
8.4
-
L-arginine pH 5.0, 37°C, Co2+-activited enzyme, K0.5 value, Hill-coefficient 2.1 Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the Co2+- and Mn2+-reconstituted enzymes exhibit cooperative mechanism of arginine hydrolysis, and undergo self-association and activation with increasing concentrations. Co2+ ions play a more important role in the local tertiary structure of the protein than Mn2+ Helicobacter pylori
Mn2+ the Co2+- and Mn2+-reconstituted enzymes exhibit cooperative mechanism of arginine hydrolysis, and undergo self-association and activation with increasing concentrations. Co2+ ions play a more important role in the local tertiary structure of the protein than Mn2+ Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
Helicobacter pylori L-ornithine + urea
-
?

Subunits

Subunits Comment Organism
dimer monomer and dimer with monomer being the major form, but the dimer is associated with higher catalytic activity, analytical gel filtration. The proportion of dimer decreases with increasing salt concentrations Helicobacter pylori
monomer monomer and dimer with monomer being the major form, but the dimer is associated with higher catalytic activity, analytical gel filtration. The proportion of dimer decreases with increasing salt concentrations Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.067
-
L-arginine pH 5.0, 37°C, Mn2+-activited enzyme Helicobacter pylori
0.28
-
L-arginine pH 5.0, 37°C, Co2+-activited enzyme Helicobacter pylori