Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
L-arginine | pH 5.0, 37°C, Mn2+-activited enzyme, K0.5 value, Hill-coefficient 1.6 | Helicobacter pylori | |
8.4 | - |
L-arginine | pH 5.0, 37°C, Co2+-activited enzyme, K0.5 value, Hill-coefficient 2.1 | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the Co2+- and Mn2+-reconstituted enzymes exhibit cooperative mechanism of arginine hydrolysis, and undergo self-association and activation with increasing concentrations. Co2+ ions play a more important role in the local tertiary structure of the protein than Mn2+ | Helicobacter pylori | |
Mn2+ | the Co2+- and Mn2+-reconstituted enzymes exhibit cooperative mechanism of arginine hydrolysis, and undergo self-association and activation with increasing concentrations. Co2+ ions play a more important role in the local tertiary structure of the protein than Mn2+ | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Helicobacter pylori | L-ornithine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | monomer and dimer with monomer being the major form, but the dimer is associated with higher catalytic activity, analytical gel filtration. The proportion of dimer decreases with increasing salt concentrations | Helicobacter pylori |
monomer | monomer and dimer with monomer being the major form, but the dimer is associated with higher catalytic activity, analytical gel filtration. The proportion of dimer decreases with increasing salt concentrations | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
L-arginine | pH 5.0, 37°C, Mn2+-activited enzyme | Helicobacter pylori | |
0.28 | - |
L-arginine | pH 5.0, 37°C, Co2+-activited enzyme | Helicobacter pylori |