Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and expressed in Bacillus subtilis as a N-terminal 6xHistagged protein | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
L-arginine | native rhArg1 | Homo sapiens | |
2.9 | - |
L-arginine | Km of pegylated rhArg1 (rhArg1-peg5,000 mw) | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35510 | - |
MALDI-TOF-MS | Homo sapiens |
35511 | - |
x * 35511, SDS-PAGE | Homo sapiens |
35560 | - |
calculated | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEP-3B cell | HBsAg-positive HCC, HB-8064, liver cancer cell line | Homo sapiens | - |
Hep-G2 cell | HBV-negative hepatoblastoma, HB-8065, liver cancer cell line | Homo sapiens | - |
hepatoma cell | HCC | Homo sapiens | - |
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
400 | - |
at 30°C, pH 8.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Homo sapiens | L-ornithine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35511, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
arginase | - |
Homo sapiens |
rhArg1 | recombinant human arginase | Homo sapiens |