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Literature summary for 3.5.3.1 extracted from
- Purification and characterization of fruit bat (Eidolon helvum, Kerr) liver arginase (2007), Int. J. Biol. Chem., 1, 11-20.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 80% residual activity at 1.5 mM | Eidolon helvum |  |
| Co2+ | 84% residual activity at 1.5 mM | Eidolon helvum | |
| Fe2+ | 78% residual activity at 1.5 mM | Eidolon helvum | |
| Mg2+ | 64% residual activity at 1.5 mM | Eidolon helvum | |
| Ni2+ | 83% residual activity at 1.5 mM | Eidolon helvum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17 | - |
L-arginine | - |
Eidolon helvum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
1 * 34000 + 1 * 52000, SDS-PAGE | Eidolon helvum |
| 52000 | - |
1 * 34000 + 1 * 52000, SDS-PAGE | Eidolon helvum |
| 80000 | - |
gel filtration | Eidolon helvum |
| 86000 | - |
SDS-PAGE | Eidolon helvum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | Eidolon helvum | - |
L-ornithine + urea | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eidolon helvum | - |
fruit bat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, CM-Sephadex column chromatography, and Bio-Gel P-100 gel filtration | Eidolon helvum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Eidolon helvum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the purified enzyme shows a specific activity of 36.7 U/mg at 37°C | Eidolon helvum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine + H2O | - |
Eidolon helvum | L-ornithine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 34000 + 1 * 52000, SDS-PAGE | Eidolon helvum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-arginine amidohydrolase | - |
Eidolon helvum |
| L-arginine ureahydrolase | - |
Eidolon helvum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Eidolon helvum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
activity drops rapidly above 60°C | Eidolon helvum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Eidolon helvum |
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