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Literature summary for 3.5.3.1 extracted from

  • Alarcon, R.; Orellana, M.S.; Neira, B.; Uribe, E.; Garcia, J.R.; Carvajal, N.
    Mutational analysis of substrate recognition by human arginase type I -agmatinase activity of the N130D variant (2006), FEBS J., 273, 5625-5631.
    View publication on PubMed
Search for more literature for 3.5.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N130D 17% activity compared to the wild type enzyme, the mutant is also active on agmatine, the N130D mutant enzyme is inactive after dialysis against EDTA and assay in the absence of Mn2+ Homo sapiens
N130Q after dialysis against EDTA and assay in the absence of Mn2+, the wild type-like N130Q mutant enzyme exhibits 50% activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA after dialysis against EDTA and assay in the absence of Mn2+, the wild type enzyme exhibits 50% activity Homo sapiens
Guanidinium chloride
-
 Homo sapiens
L-ornithine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
 L-arginine wild type enzyme, at 37°C Homo sapiens
13.3
-
 L-arginine mutant enzyme N130D, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ essentiell for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Homo sapiens
-
 L-ornithine + urea
-
 ?
additional information Homo sapiens the wild type enzyme does not hydrolyze 1-amino-4-guanidinobutane (agmatine) ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
 Homo sapiens L-ornithine + urea
-
 ?
additional information the wild type enzyme does not hydrolyze 1-amino-4-guanidinobutane (agmatine) Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
arginase type I
-
 Homo sapiens
L-arginine amidino hydrolase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33
-
 L-arginine mutant enzyme N130D, at 37°C Homo sapiens
190
-
 L-arginine wild type enzyme, at 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
 L-ornithine wild type enzyme Homo sapiens
7.8
-
 L-ornithine mutant enzyme N130D Homo sapiens
39
-
 Guanidinium chloride mutant enzyme N130D Homo sapiens
56
-
 Guanidinium chloride wild type enzyme Homo sapiens