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Literature summary for 3.5.2.B2 extracted from

  • Su, Y.; Gao, S.; Li, H.; Zheng, G.
    Enantioselective resolution of gamma-lactam utilizing a novel (+)-gamma-lactamase from Bacillus thuringiensis (2018), Proc. Biochem., 72, 96-104 .
No PubMed abstract available
Search for more literature for 3.5.2.B2

Application

Application Comment Organism
synthesis the enzyme may become a potential tool for the production of (-)-gamma-lactam because of its superior physicochemical properties and high enzyme activity Bacillus thuringiensis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) Bacillus thuringiensis

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 70% loss of activity Bacillus thuringiensis
Sn2+ 1 mM, 70% loss of activity Bacillus thuringiensis
Zn2+ 1 mM, 70% loss of activity Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
363
-
 2-azabicyclo[2.2.1]hept-5-en-3-one 37°C, pH 7.0 Bacillus thuringiensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, activity increases by 1.48 times Bacillus thuringiensis
Fe3+ 1 mM, activity increases by 1.65 times Bacillus thuringiensis
Mn2+ 1 mM, activity increases by 1.52 times Bacillus thuringiensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 calculated from sequence Bacillus thuringiensis

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis Q6HKW3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-azabicyclo[2.2.1]hept-5-en-3-one + H2O the recombinant enzyme can enantioselectively catalyze the bioresolution of racemic gamma-lactam with a high enantiomeric excess (ee) of 99.8% and enantiomeric ratio (E) above 200 Bacillus thuringiensis (-)-2-azabicyclo[2.2.1]hept-5-en-3-one + (+)-4-amino-cyclopent-2-enecarboxylic acid
-
 ?

Synonyms

Synonyms Comment Organism
SYJ322B5
-
 Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus thuringiensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 20°C: about 60% of maximal activity, 80°C: about 40% of maximal activity Bacillus thuringiensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 pH 7.0, 1 h, no loss of activity Bacillus thuringiensis
30
-
 pH 7.0, 1 h, about 10% loss of activity Bacillus thuringiensis
40
-
 pH 7.0, 1 h, about 10% loss of activity Bacillus thuringiensis
50
-
 pH 7.0, 1 h, about 65% loss of activity Bacillus thuringiensis
60
-
 pH 7.0, 1 h, about 80% loss of activity Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3145.9
-
 2-azabicyclo[2.2.1]hept-5-en-3-one 37°C, pH 7.0 Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bacillus thuringiensis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 80% of maximal activity, pH 8.0: about 50% of maximal activity Bacillus thuringiensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.1 1 h, stable Bacillus thuringiensis