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Literature summary for 3.5.2.B2 extracted from

  • Wang, J.; Zhu, J.; Wu, S.
    Immobilization on macroporous resin makes E. coli RutB a robust catalyst for production of (-) Vince lactam (2015), Appl. Microbiol. Biotechnol., 99, 4691-4700 .
    View publication on PubMed
Search for more literature for 3.5.2.B2

Application

Application Comment Organism
synthesis the transformation of an active but unstable mesophilic enzyme into a stable catalyst, achieved by immobilizing the enzyme on macroporous resins, provides a feasible approach for the preparation of optically active (-)-Vince lactam (i.e. 2-azabicyclo [2.2.1] hept-5-en-3-one), which is an important chiral synthon used as an intermediate in organic chemistry Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
C167A complete loss of gamma-lactamase activity Escherichia coli
D25A 85% loss of gamma-lactamase activity Escherichia coli
K134A complete loss of gamma-lactamase activity Escherichia coli
N73A 40% loss of gamma-lactamase activity Escherichia coli
Q27A complete loss of gamma-lactamase activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2-azabicyclo [2.2.1] hept-5-en-3-one after immobilization, the minimal substrate inhibition concentration is improved from 0.75 M to 1.5 M Escherichia coli
Ca2+ 5 mM, 95.7% inhibition Escherichia coli
Co2+ 5 mM, 80.8% inhibition Escherichia coli
Cu2+ 5 mM, 72.3% inhibition Escherichia coli
dithiothreitol 5 mM, complete inhibition Escherichia coli
Fe2+ 5 mM, 78.5% inhibition Escherichia coli
additional information the enzyme is not inhibited by 2 mM PMSF. Li+ does not disrupt enzyme activity Escherichia coli
Zn2+ 5 mM, 80.3% inhibition Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 gel filtration Escherichia coli
210000
-
 non-denaturing PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli A0A5E8GM52
-
-
Escherichia coli JM109 A0A5E8GM52
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
racemic 2-azabicyclo [2.2.1] hept-5-en-3-one + H2O
-
 Escherichia coli (+)-2-azabicyclo[2.2.1]hept-5-en-3-one + (+)-4-amino-cyclopent-2-enecarboxylic acid
-
 ?
racemic 2-azabicyclo [2.2.1] hept-5-en-3-one + H2O
-
 Escherichia coli JM109 (+)-2-azabicyclo[2.2.1]hept-5-en-3-one + (+)-4-amino-cyclopent-2-enecarboxylic acid
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 26000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
(+) gamma-lactamase
-
 Escherichia coli
RutB
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 soluble enzyme Escherichia coli
60
-
 enzyme immobilized on macroporous resin using glutaraldehyde cross-linkage Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 80% of maximal activity, 50°C: about 50% of maximal activity, soluble enzyme Escherichia coli
40 80 40°C: about 45% of maximal activity, 80°C: about 30% of maximal activity, enzyme immobilized on macroporous resin using glutaraldehyde cross-linkage Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, soluble enzyme completly loses its activity, enzyme immobilized on macroporous resin using glutaraldehyde cross-linkage loses 10% of its activity Escherichia coli
60
-
 30 min, enzyme immobilized on macroporous resin using glutaraldehyde cross-linkage loses 30% of its activity Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 soluble enzyme Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 11 pH 5.0: about 30% of maximal activity, pH 11.0: about 70% of maximal activity, soluble enzyme Escherichia coli