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Literature summary for 3.5.2.9 extracted from

  • Vetter, A.M.; Helmecke, J.; Schomburg, D.; Neumann-Schaal, M.
    The impact of pyroglutamate Sulfolobus acidocaldarius has a growth advantage over Saccharolobus solfataricus in glutamate-containing media (2019), Archaea, 2019, 3208051 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.9

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-oxo-L-proline + 2 H2O Saccharolobus solfataricus
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Saccharolobus solfataricus P2
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius DSM 639
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Saccharolobus solfataricus JCM 11322
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Saccharolobus solfataricus ATCC 35092
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius ATCC 33909
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Saccharolobus solfataricus DSM 1617
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius NBRC 15157
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius NCIMB 11770
-
 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O Sulfolobus acidocaldarius JCM 8929
-
 ADP + phosphate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97XP4 i.e. Sulfolobus solfataricus
-
Saccharolobus solfataricus ATCC 35092 Q97XP4 i.e. Sulfolobus solfataricus
-
Saccharolobus solfataricus DSM 1617 Q97XP4 i.e. Sulfolobus solfataricus
-
Saccharolobus solfataricus JCM 11322 Q97XP4 i.e. Sulfolobus solfataricus
-
Saccharolobus solfataricus P2 Q97XP4 i.e. Sulfolobus solfataricus
-
Sulfolobus acidocaldarius Q4JBP4 MW001
-
Sulfolobus acidocaldarius ATCC 33909 Q4JBP4 MW001
-
Sulfolobus acidocaldarius DSM 639 Q4JBP4 MW001
-
Sulfolobus acidocaldarius JCM 8929 Q4JBP4 MW001
-
Sulfolobus acidocaldarius NBRC 15157 Q4JBP4 MW001
-
Sulfolobus acidocaldarius NCIMB 11770 Q4JBP4 MW001
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information Sulfolobus acidocaldarius is able to grow on 24 mM 5-oxo-L-proline, reaching a maximum cell dry weight of 0.64 g/l and a maximum growth rate of 0.055/h within 96 h of cultivation Sulfolobus acidocaldarius
-
additional information Sulfolobus solfataricus does not grow at all on 24 mM 5-oxo-L-proline as a sole carbon source and in the presence of 14 mM 5-oxo-L-proline, neither as supplement nor after addition to a growing culture Saccharolobus solfataricus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-oxo-L-proline + 2 H2O
-
 Saccharolobus solfataricus ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Saccharolobus solfataricus P2 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Saccharolobus solfataricus JCM 11322 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Saccharolobus solfataricus ATCC 35092 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius ATCC 33909 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Saccharolobus solfataricus DSM 1617 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius NBRC 15157 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius NCIMB 11770 ADP + phosphate + L-glutamate
-
 ?
ATP + 5-oxo-L-proline + 2 H2O
-
 Sulfolobus acidocaldarius JCM 8929 ADP + phosphate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
5-oxoprolinase
-
 Saccharolobus solfataricus
5-oxoprolinase
-
 Sulfolobus acidocaldarius
oplA
-
 Sulfolobus acidocaldarius
SSO1667
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Saccharolobus solfataricus
65
-
 assay at Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Saccharolobus solfataricus
6.5
-
 assay at Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharolobus solfataricus
ATP
-
 Sulfolobus acidocaldarius

General Information

General Information Comment Organism
malfunction 5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death Saccharolobus solfataricus
metabolism comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures Saccharolobus solfataricus
metabolism comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures Sulfolobus acidocaldarius
additional information glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction Sulfolobus acidocaldarius
additional information glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction Saccharolobus solfataricus
physiological function 5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate Sulfolobus acidocaldarius
physiological function 5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate Saccharolobus solfataricus