Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-oxo-L-proline + 2 H2O | Saccharolobus solfataricus | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Saccharolobus solfataricus P2 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius DSM 639 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Saccharolobus solfataricus JCM 11322 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Saccharolobus solfataricus ATCC 35092 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius ATCC 33909 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Saccharolobus solfataricus DSM 1617 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius NBRC 15157 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius NCIMB 11770 | - |
ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | Sulfolobus acidocaldarius JCM 8929 | - |
ADP + phosphate + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97XP4 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus ATCC 35092 | Q97XP4 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus DSM 1617 | Q97XP4 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus JCM 11322 | Q97XP4 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus P2 | Q97XP4 | i.e. Sulfolobus solfataricus | - |
Sulfolobus acidocaldarius | Q4JBP4 | MW001 | - |
Sulfolobus acidocaldarius ATCC 33909 | Q4JBP4 | MW001 | - |
Sulfolobus acidocaldarius DSM 639 | Q4JBP4 | MW001 | - |
Sulfolobus acidocaldarius JCM 8929 | Q4JBP4 | MW001 | - |
Sulfolobus acidocaldarius NBRC 15157 | Q4JBP4 | MW001 | - |
Sulfolobus acidocaldarius NCIMB 11770 | Q4JBP4 | MW001 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Sulfolobus acidocaldarius is able to grow on 24 mM 5-oxo-L-proline, reaching a maximum cell dry weight of 0.64 g/l and a maximum growth rate of 0.055/h within 96 h of cultivation | Sulfolobus acidocaldarius | - |
additional information | Sulfolobus solfataricus does not grow at all on 24 mM 5-oxo-L-proline as a sole carbon source and in the presence of 14 mM 5-oxo-L-proline, neither as supplement nor after addition to a growing culture | Saccharolobus solfataricus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-oxo-L-proline + 2 H2O | - |
Saccharolobus solfataricus | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Saccharolobus solfataricus P2 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius DSM 639 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Saccharolobus solfataricus JCM 11322 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Saccharolobus solfataricus ATCC 35092 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius ATCC 33909 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Saccharolobus solfataricus DSM 1617 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius NBRC 15157 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius NCIMB 11770 | ADP + phosphate + L-glutamate | - |
? | |
ATP + 5-oxo-L-proline + 2 H2O | - |
Sulfolobus acidocaldarius JCM 8929 | ADP + phosphate + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-oxoprolinase | - |
Saccharolobus solfataricus |
5-oxoprolinase | - |
Sulfolobus acidocaldarius |
oplA | - |
Sulfolobus acidocaldarius |
SSO1667 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
65 | - |
assay at | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Saccharolobus solfataricus |
6.5 | - |
assay at | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharolobus solfataricus | |
ATP | - |
Sulfolobus acidocaldarius |
General Information | Comment | Organism |
---|---|---|
malfunction | 5-oxo-L-proline is a competitive inhibitor for glutamate transport in Sulfolobus solfataricus. The growth inhibiting effect of 5-oxo-L-proline on the cell culture is not only due to the loss of available carbon, because its addition to a growing culture can lead to cell death | Saccharolobus solfataricus |
metabolism | comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures | Saccharolobus solfataricus |
metabolism | comparison of the effect of 5-oxoproline on the growth of Sulfolobus solfataricus and the closely related crenarchaeon Sulfolobus acidocaldarius. Sulfolobus solfataricus shows intracellular accumulation of 5-oxoproline and crude cell extract assays show a less effective degradation of 5-oxoproline. Sulfolobus acidocaldarius seems to be less versatile regarding carbohydrates and prefers peptidolytic growth compared to Sulfolobus solfataricus. Concludingly, Sulfolobus acidocaldarius exhibits a more efficient utilization of 5-oxoproline and is not inhibited by this compound, making it a better candidate for applications with glutamate-containing media at high temperatures | Sulfolobus acidocaldarius |
additional information | glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to 5-oxoproline-induced growth restriction | Sulfolobus acidocaldarius |
additional information | glutamate is spontaneously converted into 5-oxoproline in a pH range of pH 2 to 3.5 and at temperatures above room temperature. This makes many thermoacidophiles, like, for example, Sulfolobus solfataricus, less suitable for a number of biotechnological approaches due to pyroglutamate-induced growth restriction | Saccharolobus solfataricus |
physiological function | 5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline to glutamate | Sulfolobus acidocaldarius |
physiological function | 5-oxoprolinase catalyses the ATP-dependent conversion of 5-oxoproline, i.e. pyroglutamate, to glutamate | Saccharolobus solfataricus |