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Literature summary for 3.5.2.9 extracted from

  • Mochizuki, K.
    Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4-carboxylic acid (1999), Arch. Microbiol., 172, 182-185.
    View publication on PubMed
Search for more literature for 3.5.2.9

Activating Compound

Activating Compound Comment Organism Structure
NH2OH-HCl 1 mM, relative activity 109% Paecilomyces variotii

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Paecilomyces variotii
CuSO4
-
 Paecilomyces variotii
N-bromosuccinimide
-
 Paecilomyces variotii
N-ethylmaleimide
-
 Paecilomyces variotii
N-ethylmaleimide
-
 Rattus norvegicus
p-hydroxymercuribenzoate
-
 Paecilomyces variotii
p-hydroxymercuribenzoate
-
 Rattus norvegicus
ZnCl2
-
 Paecilomyces variotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Rattus norvegicus
0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Pseudomonas putida
0.005
-
 5-oxo-L-proline
-
 Rattus norvegicus
0.043
-
 5-oxo-L-proline
-
 Pseudomonas putida
0.07
-
 5-oxoproline
-
 Alcaligenes sp.
0.091
-
 ATP 5-oxo-L-proline as substrate Paecilomyces variotii
0.17
-
 L-2-Oxothiazolidine-4-carboxylate
-
 Paecilomyces variotii
0.21
-
 ATP L-2-oxothiazolidine-4-carboxylate as substrate Paecilomyces variotii

Metals/Ions

Metals/Ions Comment Organism Structure
FeSO4 1 mM , relative activity 113% Paecilomyces variotii
K+
-
 Rattus norvegicus
K+
-
 Paecilomyces variotii
K+
-
 Pseudomonas putida
Mg2+
-
 Rattus norvegicus
Mg2+
-
 Paecilomyces variotii
Mn2+
-
 Rattus norvegicus
Mn2+ 1 mM MnCl2, relative activity 144% Paecilomyces variotii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 2 * 140000, SDS-PAGE Rattus norvegicus
140000
-
 2 * 140000, SDS-PAGE Paecilomyces variotii
260000
-
 gel filtration Paecilomyces variotii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Paecilomyces variotii
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Pseudomonas putida
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Alcaligenes sp.
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Paecilomyces variotii F-1
-
 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP Alcaligenes sp. F-137
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
-
-
Alcaligenes sp. F-137
-
-
-
Paecilomyces variotii
-
-
-
Paecilomyces variotii F-1
-
-
-
Pseudomonas putida
-
-
-
Rattus norvegicus
-
 rat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.69
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Paecilomyces variotii L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Pseudomonas putida L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Alcaligenes sp. L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Paecilomyces variotii F-1 L-glutamate + ADP + phosphate
-
 ?
5-oxo-L-proline + H2O + ATP
-
 Alcaligenes sp. F-137 L-glutamate + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Paecilomyces variotii L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Alcaligenes sp. L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Paecilomyces variotii F-1 L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Alcaligenes sp. F-137 L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
additional information
-
 Pseudomonas putida ?
-
 ?
additional information CTP, TTP and UTP are no substrates Paecilomyces variotii ?
-
 ?
additional information CTP, TTP and UTP are no substrates Paecilomyces variotii F-1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 140000, SDS-PAGE Rattus norvegicus
dimer 2 * 140000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paecilomyces variotii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55
-
 Paecilomyces variotii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 64% initial activity retained at 60°C, loses all initial activity at 70°C Paecilomyces variotii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Paecilomyces variotii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 most stable at pH 7.5, loses activity rapidly below pH 5.5 and above pH 9 Paecilomyces variotii