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Literature summary for 3.5.2.9 extracted from
- 5-Oxo-L-Prolinase from rat kidney (1985), Methods Enzymol., 113, 445-451.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-imidazolidone-4-carboxylate | 5-oxoproline analog, stimulates rapid ATP cleavage, but is not hydrolyzed itself | Rattus norvegicus |  |
| 2-piperidone-6-carboxylate | stimulates ATP cleavage | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
5-oxo-L-proline | - |
Rattus norvegicus | |
| 0.13 | - |
ITP | - |
Rattus norvegicus | |
| 0.13 | - |
ATP | measured in absence of 5-oxoproline | Rattus norvegicus | |
| 0.17 | - |
ATP | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 142000 | - |
2 * 142000, SDS-PAGE | Rattus norvegicus |
| 325000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | Rattus norvegicus | conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations | L-glutamate + ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 87 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-oxo-L-proline + H2O + ATP | - |
Rattus norvegicus | L-glutamate + ADP + phosphate | - |
ir | |
| 5-oxo-L-proline + H2O + ATP | conversion essentially irreversible, very slow reversal can be shown by measurement of ATP formation in presence of high glutamate concentrations | Rattus norvegicus | L-glutamate + ADP + phosphate | - |
ir | |
| alpha-hydroxyglutarate lactone + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| ITP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| L-2-oxothiazolidine-4-carboxylate + H2O + ATP | - |
Rattus norvegicus | L-cysteine + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 142000, SDS-PAGE | Rattus norvegicus |
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