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Literature summary for 3.5.2.6 extracted from

  • Kusada, H.; Tamaki, H.; Kamagata, Y.; Hanada, S.; Kimura, N.
    A novel quorum-quenching N-acylhomoserine lactone acylase from Acidovorax sp. strain MR-S7 mediates antibiotic resistance (2017), Appl. Environ. Microbiol., 83, e00080-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene macQ, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Origami 2(DE3). Recombinant plasmid (pMQ28) confers 2 to 16fold higher MIC values toward all six beta-lactam antibiotics in Escherichia coli Origami 2(DE3) than the strain with a control vector. This indicates that MacQ confers multiple beta-lactam antibiotic resistance on a host strain by hydrolyzing beta-lactam antibiotics Acidovorax sp. MR-S7

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the first 24 residues of MacQ are predicted to be a signal sequence Acidovorax sp. MR-S7
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
sequence calculation Acidovorax sp. MR-S7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amoxicillin + H2O Acidovorax sp. MR-S7
-
(2R,4S)-2-[[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ampicillin + H2O Acidovorax sp. MR-S7
-
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
carbenicillin + H2O Acidovorax sp. MR-S7
-
(2R,4S)-2-((R)-carboxy[2-carboxy(phenyl)acetamido]methyl)-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefadroxil + H2O Acidovorax sp. MR-S7
-
(2R)-2-[((R)-([(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino)carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cephalexin + H2O Acidovorax sp. MR-S7
-
?
-
?
additional information Acidovorax sp. MR-S7 enzyme MacQ has the bifunctional capacity to degrade both N-acylhomoserine lactone (N-acylhomoserine lactone acylase, EC 3.5.1.97) and beta-lactam antibiotics by deacylation activity. MacQ is able to inactivate a variety of beta-lactams, including penicillin derivatives (penicillin G, ampicillin, amoxicillin, and carbenicillin) and cephalosporin derivatives (cephalexin and cefadroxil) ?
-
-
penicillin G + H2O Acidovorax sp. MR-S7
-
(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Organism

Organism UniProt Comment Textmining
Acidovorax sp. MR-S7 AB702957 isolated from activated sludge in a penicillin G production wastewater treatment system
-

Purification (Commentary)

Purification (Comment) Organism
gene macQ, recombinant His-tagged enzyme from Escherichia coli strain Origami 2(DE3) by nickel affinity chromatography Acidovorax sp. MR-S7

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amoxicillin + H2O
-
Acidovorax sp. MR-S7 (2R,4S)-2-[[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ampicillin + H2O
-
Acidovorax sp. MR-S7 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
carbenicillin + H2O
-
Acidovorax sp. MR-S7 (2R,4S)-2-((R)-carboxy[2-carboxy(phenyl)acetamido]methyl)-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefadroxil + H2O
-
Acidovorax sp. MR-S7 (2R)-2-[((R)-([(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino)carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cephalexin + H2O
-
Acidovorax sp. MR-S7 ?
-
?
additional information enzyme MacQ has the bifunctional capacity to degrade both N-acylhomoserine lactone (N-acylhomoserine lactone acylase, EC 3.5.1.97) and beta-lactam antibiotics by deacylation activity. MacQ is able to inactivate a variety of beta-lactams, including penicillin derivatives (penicillin G, ampicillin, amoxicillin, and carbenicillin) and cephalosporin derivatives (cephalexin and cefadroxil) Acidovorax sp. MR-S7 ?
-
-
penicillin G + H2O
-
Acidovorax sp. MR-S7 (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 20000, alpha-subunit, + 1 * 62000, beta-subunit, SDS-PAGE, recombinant enzyme Acidovorax sp. MR-S7

Synonyms

Synonyms Comment Organism
MacQ
-
Acidovorax sp. MR-S7
More also see for EC 3.5.1.97 Acidovorax sp. MR-S7

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Acidovorax sp. MR-S7

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Acidovorax sp. MR-S7

General Information

General Information Comment Organism
physiological function MacQ is a bifunctional enzyme that confers both quorum quenching and antibiotic resistance on strain MRS7. The enzyme is capable of degrading not only various N-acylhomoserine lactones (AHLs) derivatives but also multiple beta-lactam antibiotics by deacylation activities. Recombinant MacQ confers multiple beta-lactam antibiotic resistance on an Escherichia coli host strain by hydrolyzing beta-lactam antibiotics. The N-acylhomoserine lactone acylase (AHL acylase) activity is responsible for disrupting cell-cell communication (quorum sensing) in bacteria Acidovorax sp. MR-S7