Cloned (Comment) | Organism |
---|---|
gene macQ, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain Origami 2(DE3). Recombinant plasmid (pMQ28) confers 2 to 16fold higher MIC values toward all six beta-lactam antibiotics in Escherichia coli Origami 2(DE3) than the strain with a control vector. This indicates that MacQ confers multiple beta-lactam antibiotic resistance on a host strain by hydrolyzing beta-lactam antibiotics | Acidovorax sp. MR-S7 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the first 24 residues of MacQ are predicted to be a signal sequence | Acidovorax sp. MR-S7 | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
sequence calculation | Acidovorax sp. MR-S7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + H2O | Acidovorax sp. MR-S7 | - |
(2R,4S)-2-[[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | Acidovorax sp. MR-S7 | - |
(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | Acidovorax sp. MR-S7 | - |
(2R,4S)-2-((R)-carboxy[2-carboxy(phenyl)acetamido]methyl)-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefadroxil + H2O | Acidovorax sp. MR-S7 | - |
(2R)-2-[((R)-([(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino)carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalexin + H2O | Acidovorax sp. MR-S7 | - |
? | - |
? | |
additional information | Acidovorax sp. MR-S7 | enzyme MacQ has the bifunctional capacity to degrade both N-acylhomoserine lactone (N-acylhomoserine lactone acylase, EC 3.5.1.97) and beta-lactam antibiotics by deacylation activity. MacQ is able to inactivate a variety of beta-lactams, including penicillin derivatives (penicillin G, ampicillin, amoxicillin, and carbenicillin) and cephalosporin derivatives (cephalexin and cefadroxil) | ? | - |
- |
|
penicillin G + H2O | Acidovorax sp. MR-S7 | - |
(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax sp. MR-S7 | AB702957 | isolated from activated sludge in a penicillin G production wastewater treatment system | - |
Purification (Comment) | Organism |
---|---|
gene macQ, recombinant His-tagged enzyme from Escherichia coli strain Origami 2(DE3) by nickel affinity chromatography | Acidovorax sp. MR-S7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + H2O | - |
Acidovorax sp. MR-S7 | (2R,4S)-2-[[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Acidovorax sp. MR-S7 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
Acidovorax sp. MR-S7 | (2R,4S)-2-((R)-carboxy[2-carboxy(phenyl)acetamido]methyl)-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefadroxil + H2O | - |
Acidovorax sp. MR-S7 | (2R)-2-[((R)-([(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino)carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalexin + H2O | - |
Acidovorax sp. MR-S7 | ? | - |
? | |
additional information | enzyme MacQ has the bifunctional capacity to degrade both N-acylhomoserine lactone (N-acylhomoserine lactone acylase, EC 3.5.1.97) and beta-lactam antibiotics by deacylation activity. MacQ is able to inactivate a variety of beta-lactams, including penicillin derivatives (penicillin G, ampicillin, amoxicillin, and carbenicillin) and cephalosporin derivatives (cephalexin and cefadroxil) | Acidovorax sp. MR-S7 | ? | - |
- |
|
penicillin G + H2O | - |
Acidovorax sp. MR-S7 | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 20000, alpha-subunit, + 1 * 62000, beta-subunit, SDS-PAGE, recombinant enzyme | Acidovorax sp. MR-S7 |
Synonyms | Comment | Organism |
---|---|---|
MacQ | - |
Acidovorax sp. MR-S7 |
More | also see for EC 3.5.1.97 | Acidovorax sp. MR-S7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Acidovorax sp. MR-S7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Acidovorax sp. MR-S7 |
General Information | Comment | Organism |
---|---|---|
physiological function | MacQ is a bifunctional enzyme that confers both quorum quenching and antibiotic resistance on strain MRS7. The enzyme is capable of degrading not only various N-acylhomoserine lactones (AHLs) derivatives but also multiple beta-lactam antibiotics by deacylation activities. Recombinant MacQ confers multiple beta-lactam antibiotic resistance on an Escherichia coli host strain by hydrolyzing beta-lactam antibiotics. The N-acylhomoserine lactone acylase (AHL acylase) activity is responsible for disrupting cell-cell communication (quorum sensing) in bacteria | Acidovorax sp. MR-S7 |